Department of Surgery and Bucco-Maxillofacial Traumatology and Periodontology, Ribeirão Preto School of Dentistry, University of São Paulo - USP, Ribeirão Preto, SP, Brazil.
Federal University of Ceará - UFC, Sobral, Ceará, Brazil.
Arch Oral Biol. 2018 Apr;88:1-9. doi: 10.1016/j.archoralbio.2018.01.005. Epub 2018 Jan 9.
The purpose of this study was to evaluate whether local administration of TIL could influence the expression of the inflammatory mediators IL-1β, TNF-α, MMP-8 and COX-2 in rats with experimental periodontitis (EP).
Twenty-four adult male rats (Rattus norvegicus, albinus, Wistar) were assigned to groups C, EP, EP-TIL (CControl group, EP-Periodontitis groups). On EP groups, a ligature was placed around maxillary 2nd molars on day 1. On group EP-TIL, 20 μL of TIL solution (1 mg/kg body weight) was injected into the subperiosteal palatal area adjacent to the maxillary 2nd molar every other day until euthanasia (day 11). Alveolar bone loss was morphometrically analyzed. mRNA expressions of IL-1β, TNF-α, MMP-8 and COX-2 were assessed by qPCR. IL-1β, TNF-α, MMP-8 and COX-2 were immunohistochemically analyzed. Data were analyzed statistically.
Group EP-TIL presented reduced alveolar bone loss when compared with group EP (p < 0.05). Group EP-TIL presented decreased mRNA expressions of IL-1β, TNF-α, MMP-8 and COX-2 and reduced immunolabeling of IL-1β, TNF-α and MMP-8 when compared with group EP (p < 0.05). No differences regarding the immunolabeling of COX-2 were found when group EP-TIL was compared with the other groups (p > 0.05).
Within the limits of this study, it can be concluded that local administration of TIL downregulates important mediators involved in periodontal tissue destruction in ligature-induced periodontitis in rats.
本研究旨在评估 TIL 的局部给药是否会影响实验性牙周炎（EP）大鼠中炎症介质 IL-1β、TNF-α、MMP-8 和 COX-2 的表达。
将 24 只成年雄性大鼠（Rattus norvegicus、白化、Wistar）分为 C、EP、EP-TIL（C-对照组、EP-牙周炎组）。在 EP 组，第 1 天在上颌第 2 磨牙周围放置结扎线。在 EP-TIL 组，每隔一天在上颌第 2 磨牙附近的骨膜下腭区注射 20 μL 的 TIL 溶液（1mg/kg 体重），直至安乐死（第 11 天）。通过形态计量学分析牙槽骨丧失。通过 qPCR 评估 IL-1β、TNF-α、MMP-8 和 COX-2 的 mRNA 表达。通过免疫组织化学分析 IL-1β、TNF-α、MMP-8 和 COX-2。对数据进行统计学分析。
与 EP 组相比，EP-TIL 组的牙槽骨丧失减少（p<0.05）。与 EP 组相比，EP-TIL 组的 IL-1β、TNF-α、MMP-8 和 COX-2 的 mRNA 表达降低，IL-1β、TNF-α 和 MMP-8 的免疫标记减少（p<0.05）。与其他组相比，EP-TIL 组的 COX-2 免疫标记无差异（p>0.05）。
在本研究范围内，可以得出结论，TIL 的局部给药可下调结扎诱导的牙周炎大鼠牙周组织破坏中涉及的重要介质。
