Vannini Andrea, Paoli Luca, Vichi Marco, Bačkor Martin, Bačkorová Miriam, Loppi Stefano
Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100, Siena, Italy.
Department of Botany, Pavol Jozef Šafárik University, Manesova 23, Košice, 041 67, Slovak Republic.
Bull Environ Contam Toxicol. 2018 Mar;100(3):430-437. doi: 10.1007/s00128-017-2266-4. Epub 2018 Jan 16.
This study investigated the occurrence of toxicity, expressed as damage to the photosynthetic apparatus, in the aquatic fern Azolla filiculoides and the lichen Xanthoria parietina following treatments with diclofenac at different concentrations (0.1, 1, 10 and 100 mg/L) and different exposure times (24, 48, 72 and 240 h). Measurements of photosynthetic efficiency, chlorophyll content and chlorophyll degradation indicated dose- and time-dependent toxicity, since significant differences with control samples as well as among treatments, emerged mainly for the highest concentration (100 mg/L) and the longest time (240 h). In addition, also the mycobiont of the lichen X. parietina showed similar toxic effects, expressed as ergosterol content. The absence of relevant alterations at the lowest concentration (0.1 mg/L) suggested a very limited susceptibility of these species to environmentally relevant levels of this pharmaceutical.
本研究调查了在用不同浓度（0.1、1、10和100毫克/升）双氯芬酸处理以及不同暴露时间（24、48、72和240小时）后，水生蕨类植物细叶满江红和地衣石黄衣中以光合器官损伤表示的毒性发生情况。光合效率、叶绿素含量和叶绿素降解的测量表明存在剂量和时间依赖性毒性，因为与对照样品以及各处理之间的显著差异主要出现在最高浓度（100毫克/升）和最长时间（240小时）时。此外，地衣石黄衣的共生菌也表现出类似的毒性效应，以麦角甾醇含量表示。在最低浓度（0.1毫克/升）下未出现相关变化，这表明这些物种对该药物与环境相关水平的敏感性非常有限。
