Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, Henan, China.
Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, Henan, China.
Int J Biol Macromol. 2018 May;111:623-631. doi: 10.1016/j.ijbiomac.2018.01.022. Epub 2018 Jan 11.
Long non-coding RNAs (lncRNAs) may serve as miRNA sponges to modulate the expressions of miRNA target genes. LncRNA X-inactive specific transcript (XIST) has been demonstrated to be upregulated and act as an oncogene in non-small cell lung cancer (NSCLC). However, the sponge role of XIST in NSCLC progression remains largely unknown. In this study, we demonstrated that XIST was substantially upregulated and miR-137 was aberrantly downregulated in NSCLC tissues and cells. XIST was identified to function as a competitive endogenous RNA (ceRNA) for miR-137 to promote NSCLC cell viability and invasion. Additionally, our results suggested that miR-137 targeted the 3'UTR of paxillin (PXN) to suppress NSCLC cell viability and invasion. Meanwhile, miR-137 was negatively correlated with PXN expression while XIST was positively correlated with PXN expression. More importantly, XIST positively regulated PXN levels by sponging miR-137 in vitro and in vivo. Collectively, our study provided the evidence for the cross-talk between XIST, miR-137, and PXN, shedding light on the therapy for NSCLC.
长非编码 RNA（lncRNA）可作为 miRNA 海绵来调节 miRNA 靶基因的表达。X 失活特异性转录本（XIST）lncRNA 已被证明在非小细胞肺癌（NSCLC）中上调并作为癌基因发挥作用。然而，XIST 在 NSCLC 进展中的海绵作用在很大程度上尚不清楚。在本研究中，我们证明 XIST 在 NSCLC 组织和细胞中明显上调，miR-137 异常下调。XIST 被鉴定为 miR-137 的竞争性内源 RNA（ceRNA），可促进 NSCLC 细胞活力和侵袭。此外，我们的结果表明，miR-137 通过靶向锚蛋白（PXN）的 3'UTR 来抑制 NSCLC 细胞活力和侵袭。同时，miR-137 与 PXN 表达呈负相关，而 XIST 与 PXN 表达呈正相关。更重要的是，XIST 在体外和体内通过海绵吸附 miR-137 来正向调节 PXN 水平。总之，我们的研究提供了 XIST、miR-137 和 PXN 之间相互作用的证据，为 NSCLC 的治疗提供了新的思路。
