GAEC1 mRNA 和蛋白的表达及其与结直肠腺癌患者临床病理参数的关系。
Expression of GAEC1 mRNA and protein and its association with clinical and pathological parameters of patients with colorectal adenocarcinoma.
机构信息
Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia.
Cancer Molecular Pathology, School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia; School of Medical Science, Griffith University, Gold Coast, Queensland, Australia.
出版信息
Exp Mol Pathol. 2018 Feb;104(1):71-75. doi: 10.1016/j.yexmp.2018.01.004. Epub 2018 Jan 11.
AIM
GAEC1 (Gene amplified in esophageal cancer 1) is an oncogene with key regulatory roles in the pathogenesis of oesophageal and colorectal carcinomas. The aim of this study was to investigate expression profiles and clinicopathological significance of GAEC1 mRNA and protein in patients with colorectal carcinomas.
METHOD
Matched cancer and non-cancer fresh frozen tissues were prospectively collected from 80 patients diagnosed with colorectal adenocarcinoma (39 men and 41 women). The tissues were sectioned for RNA extraction and cDNA conversion and quantified by a real-time polymerase chain reaction. GAEC1 protein expression was analysed by immunohistochemistry using a custom made GAEC1 antibody.
RESULT
GAEC1 mRNA was upregulated in majority (52%, n=42/80) of the colorectal carcinomas when compared to the matched non-neoplastic tissues. High expression of GAEC1 mRNA as correlated with patients of younger age (p=0.008), with lower grade carcinoma (p=0.028), presence of synchronous adenocarcinomas (p=0.034) and without any associated adenomas (p=0.047). In addition, patients with high GAEC1 mRNA overexpression had a shorter survival time. Furthermore, high GAEC1 protein expression was noted among patients having perforated colorectal carcinoma (p=0.04).
CONCLUSION
The high expression of GAEC1 mRNA/protein as well as its correlation with multiple clinicopathological characteristics in patients with colorectal carcinoma strongly suggests that GAEC1 is a key regulator in the initiation of colorectal carcinogenesis.
目的
GAEC1（食管癌基因扩增 1）是一种癌基因，在食管癌和结直肠癌的发病机制中具有关键的调节作用。本研究旨在探讨 GAEC1mRNA 和蛋白在结直肠癌患者中的表达谱及临床病理意义。
方法
前瞻性收集 80 例结直肠腺癌患者（男 39 例，女 41 例）的配对癌组织和非癌组织新鲜冷冻组织。对组织进行 RNA 提取和 cDNA 转换，并通过实时聚合酶链反应定量。使用定制的 GAEC1 抗体通过免疫组织化学分析 GAEC1 蛋白表达。
结果
与匹配的非肿瘤组织相比，大多数（52%，n=42/80）结直肠癌中 GAEC1mRNA 上调。GAEC1mRNA 高表达与患者年龄较小（p=0.008）、肿瘤分级较低（p=0.028）、存在同步腺癌（p=0.034）和无相关腺瘤（p=0.047）相关。此外，GAEC1mRNA 高表达的患者生存时间较短。此外，GAEC1 蛋白高表达见于穿孔性结直肠癌患者（p=0.04）。
结论
GAEC1mRNA/蛋白高表达及其与结直肠癌患者多种临床病理特征的相关性强烈提示 GAEC1 是结直肠发生的关键调节因子。
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