Catania Salvatore, Carnaccini Silvia, Mainenti Marta, Moronato Maria Luisa, Gobbo Federica, Calogero Terregino
A Istituto Zooprofilattico Sperimentale di Padova, Viale dell'Università 10, Legnaro, PD, 35020, Italy.
B Poultry Diagnostic and Research Center, The University of Georgia, 953 College Station Road, Athens, GA 30602.
Avian Dis. 2017 Dec;61(4):531-535. doi: 10.1637/11713-071417-Case.1.
Poxvirus was the causative agent of two unusual outbreaks of proliferative glossitis in canary ( Serinus canaria forma domestica) breeders in the Northern Italy. A total of 45, 7-9-mo-old canaries were submitted in fair postmortem conditions to the Istituto Zooprofilattico Sperimentale delle Venezie at the beginning of November 2005 for diagnostic investigation. Birds belonged to two unrelated and geographically distant aviaries in northern Italy, herein identified as Aviary A and Aviary B. The two breeder flocks had both attended the same bird exposition held at the beginning of October and started experiencing an onset of high mortality 3 wk after the show. Twelve red factor-melanin canaries from Aviary A (Mantua) and 33 dominant white and recessive white canaries from Aviary B (Vicenza) were submitted for laboratory investigations. Clinical signs were unspecific and consisted of depression, ruffling of the feathers, epistaxis, and anorexia due to decreased feed and water intake. Postmortem findings revealed a severe increase in volume, thickening, and hardening of the tongue, which had turned pinkish to dark brown. No apparent gross lesions were noticed in integumentary, respiratory, and digestive systems or other internal organs. Histopathologic evaluation of the tongue revealed severe proliferation of the stratified squamous epithelium containing numerous large eosinophilic intracytoplasmic inclusion bodies (Bollinger bodies) displacing the nuclei of the cells peripherally. Severe ulceration of the surface epithelium, fibrinoheterophilic plaque formation, and moderate basal lymphoplasmacytic infiltrations were also associated with the proliferative lesion. Poxvirus was successfully isolated from the lesions in tissue cultures but not in specific-pathogen-free chicken embryonated eggs. Typical large, brick-shaped viral particles of 300-450 nm were also observed in affected tongues by transmission electron microscopy. This is the first report of multiple outbreaks of "poxvirus glossitis" in canaries.
痘病毒是意大利北部金丝雀（Serinus canaria forma domestica）饲养者中两起异常的增殖性舌炎暴发的病原体。2005年11月初，共有45只7 - 9月龄的金丝雀在良好的尸检状态下被提交给威尼斯动物卫生实验研究所进行诊断调查。这些鸟来自意大利北部两个不相关且地理位置遥远的鸟舍，在此分别称为鸟舍A和鸟舍B。这两个饲养鸟群都参加了10月初举行的同一场鸟类展览，并在展览后3周开始出现高死亡率。来自鸟舍A（曼图亚）的12只红因子 - 黑色素金丝雀和来自鸟舍B（维琴察）的33只显性白色和隐性白色金丝雀被提交进行实验室调查。临床症状无特异性，包括精神沉郁、羽毛蓬松、鼻出血以及由于采食量和饮水量减少导致的厌食。尸检结果显示舌头体积严重增大、增厚和变硬，颜色从粉红色变为深褐色。在皮肤、呼吸和消化系统或其他内部器官未发现明显的肉眼病变。对舌头的组织病理学评估显示，分层鳞状上皮严重增生，含有大量大的嗜酸性胞质内包涵体（博林格小体），使细胞的细胞核移位到周边。表面上皮的严重溃疡、纤维蛋白 - 嗜异性斑块形成以及中度基底淋巴细胞和浆细胞浸润也与增殖性病变相关。痘病毒在组织培养中成功从病变中分离出来，但在无特定病原体的鸡胚中未分离出来。通过透射电子显微镜在受影响的舌头中也观察到了典型的300 - 450纳米大的砖形病毒颗粒。这是金丝雀中多次暴发“痘病毒舌炎”的首次报告。
