Hołda Mateusz K, Klimek-Piotrowska Wiesława, Koziej Mateusz, Tyrak Kamil, Hołda Jakub
Department of Anatomy Jagiellonian University Medical College, Kopernika 12, Kraków, Poland.
Folia Med Cracov. 2017;57(4):63-70.
To investigate the penetration depth of formaldehyde-based fixatives into cardiac muscle samples over the course of fixation.
Fixation is the essential step in anatomical studies. However, very little is known about penetration of most common fixatives into cardiac tissue.
A total of 40 heart samples were investigated. 4 study groups (n=10 in each case) were formed in such manner they differed only in concentration and type of fixative (1) - 2% formaldehyde phosphate-buffered solution (FPBS); (2) - 4% FPBS (formalin); (3) - 10% FPBS; (4) - alcoholic formalin. Samples were measured before fixation and in the following time points: 24 hours, 72 hours, 168 hours.
The penetration depth differed significantly among studied fixatives (p<0.0001). 100% penetration occurred in all samples after 72 hours in alcoholic formalin solution and after 168 hours in 10% FPBS. After alcoholic formalin fixation, the tissue is more brittle and sub-epicardial blisters were observed in some cases.
Alcoholic formalin solution is the fastest fixative among the studied ones, however it has several adverse effects on tissue structure. It was found that 10% FPBS is the best and a relatively fast fixative for cardiac morphometric studies.
研究基于甲醛的固定剂在固定过程中渗透到心肌样本中的深度。
固定是解剖学研究中的关键步骤。然而，对于最常用的固定剂渗透到心脏组织中的情况却知之甚少。
共研究了40个心脏样本。以仅固定剂浓度和类型不同的方式形成4个研究组（每组n = 10）：（1）- 2%磷酸缓冲甲醛溶液（FPBS）；（2）- 4% FPBS（福尔马林）；（3）- 10% FPBS；（4）- 酒精福尔马林。在固定前以及以下时间点对样本进行测量：24小时、72小时、168小时。
在所研究的固定剂中，渗透深度存在显著差异（p < 0.0001）。在酒精福尔马林溶液中72小时后以及在10% FPBS中168小时后，所有样本均达到100%渗透。酒精福尔马林固定后，组织更脆，在某些情况下观察到心外膜下出现水泡。
酒精福尔马林溶液在所研究的固定剂中是最快的，但对组织结构有若干不利影响。发现10% FPBS是心脏形态计量学研究的最佳且相对较快的固定剂。
