Souza Marcos Paulo Almeida, Lima Fernanda Macedo da Silva, Muniz Igor Pereira, Pereira Ítalo Sousa, Sousa Larissa Rodrigues de Oliveira, Galantini Maria Poliana Leite, Santos Denisar Palmito Dos, Figueiredo Tiana Baqueiro, Silva Robson Amaro Augusto da
Department of Biointegration, Histopathology Laboratory, Multidisciplinary Institute for Health, Campus Anísio Teixeira, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil AND Department of Health, School Ruy Barbosa, Devry Group, Salvador, Bahia, Brazil.
Department of Biointegration, Histopathology Laboratory, Multidisciplinary Institute for Health, Campus Anísio Teixeira, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil.
Iran J Allergy Asthma Immunol. 2017 Dec;16(6):525-536.
Asthma is a chronic inflammation of the airways affecting over 300 million people worldwide. As in the autoimmune diseases, it is well described that women are the most affected by asthma. The higher number of women presenting this pathology suggests the involvement of female sex hormones in the construction of the allergic immune response. Female Balb / c mice were used for the experiments. Thirty-eight animals were separated into four groups: OVX-Ova; Sham-Ova; OVX-Sal; Sham-Sal. Then animals underwent acute allergic induction protocol by Ovalbulmin (OVA). Ovariectomized animals showed greater number of leukocytes in bronchoalveolar lavage (BAL) and elevated white blood cells recruitment to the lung environment observed by histological analysis. There was a significant increase of eosinophils and mast cells in inflammatory sites at pulmonary tissue. The relative uterine and body weight were lower in ovariectomized animals and higher in Sham mice, respectively. Moreover, the lack of the sex hormones induced an increase in interleukin (IL)-4 and titers of immunoglobulin G1 (IgG1) antibodies. However, increased production of IL-17A was only observed in Sham animals. Altogether, data this study suggest that ovariectomy induces the formation of a stronger Th2 response in allergic animal. However, the immune processes involved in the allergic response in females currently remain unclear.
哮喘是一种影响全球超过3亿人的气道慢性炎症。与自身免疫性疾病一样，有充分描述表明女性受哮喘影响最为严重。出现这种病症的女性数量较多，提示女性性激素参与了过敏性免疫反应的构建。实验使用雌性Balb / c小鼠。38只动物被分为四组：去卵巢-卵清蛋白组（OVX-Ova）；假手术-卵清蛋白组（Sham-Ova）；去卵巢-生理盐水组（OVX-Sal）；假手术-生理盐水组（Sham-Sal）。然后通过卵清蛋白（OVA）对动物进行急性过敏诱导方案。通过组织学分析观察到，去卵巢动物支气管肺泡灌洗（BAL）中的白细胞数量更多，并且白细胞向肺组织的募集增加。肺组织炎症部位的嗜酸性粒细胞和肥大细胞显著增加。去卵巢动物的相对子宫和体重分别较低，而假手术小鼠的相对子宫和体重较高。此外，缺乏性激素会导致白细胞介素（IL）-4和免疫球蛋白G1（IgG1）抗体滴度增加。然而，仅在假手术动物中观察到IL-17A的产生增加。总之，本研究数据表明去卵巢会在过敏性动物中诱导更强的Th2反应形成。然而，目前女性过敏反应中涉及的免疫过程仍不清楚。
