Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS , Yantai, Shandong 264003, People's Republic of China.
Department of Environmental Sciences, Key Laboratory of Watershed Science and Health of Zhejiang Province, Wenzhou Medical University , Wenzhou 325035, People's Republic of China.
Anal Chem. 2018 Feb 6;90(3):1734-1739. doi: 10.1021/acs.analchem.7b03522. Epub 2018 Jan 25.
A polymeric membrane ion-selective electrode (ISE) is typically designed for the determination of one specific ion using a conventional method. In this work, we demonstrate a simple, versatile, and sensitive platform for simultaneous detection of two molecules with a single ISE. Under a series of periodic galvanostatic polarization, a solid-contact ISE without ion exchanger properties under zero-current conditions has been successfully used for simultaneous detection of two opposite charged ions with high sensitivity, good selectivity, and fast reversibility. By integration of biorecognition elements with the potentiometric measurement, highly sensitive and selective detection of a broad range of different molecular targets can be predicted. As a proof of concept, a potentiometric genosensor based on magnetic beads-enzyme sandwich assay has been designed for sensitive and selective detection of pathogenic bacteria Escherichia coli O157:H7 and Staphylococcus aureus. Under optimal conditions, two bacteria nucleic acid sequences can be detected simultaneously with high sensitivity and good selectivity by using a single solid-contact potentiometric ISE. The detection limits of Escherichia coli O157:H7 DNA and Staphylococcus aureus DNA are 120 and 54 fM (3σ), respectively. Because of its simplicity, this potentiometric technique based on ISE can be an attractive tool or detector to perform two analyte measurements.
聚合膜离子选择性电极（ISE）通常采用传统方法设计用于测定一种特定离子。在这项工作中，我们展示了一种简单、通用且灵敏的平台，可通过单个 ISE 同时检测两种分子。在一系列周期性恒电流极化下，在零电流条件下不具有离子交换剂性质的固态接触 ISE 已成功用于同时高灵敏度、良好选择性和快速可逆性地检测两种带相反电荷的离子。通过将生物识别元件与电位测量相结合，可以预测对广泛不同分子靶标的高灵敏度和选择性检测。作为概念验证，设计了基于磁珠-酶夹心测定法的电位基因传感器，用于灵敏和选择性检测致病性细菌大肠杆菌 O157:H7 和金黄色葡萄球菌。在最佳条件下，通过使用单个固态接触电位 ISE 可以同时高灵敏度和良好选择性地检测两种细菌核酸序列。大肠杆菌 O157:H7 DNA 和金黄色葡萄球菌 DNA 的检测限分别为 120 和 54 fM（3σ）。由于其简单性，这种基于 ISE 的电位技术可以成为执行两种分析物测量的有吸引力的工具或检测器。
