Adépo Jean-Baptiste Aholia, Manda Pierre, Ngbé Jean Verdier, Diakité Aïssata, Tigori Sangaré Béatrice, Dano Sébastien Djédjé
a Laboratory of Toxicology and Agro-Industrial Hygiene, Faculty of Pharmaceutical and Biological Sciences , Félix Houphouët-Boigny University , Abidjan , BP V 34 , Côte d'Ivoire.
Drug Chem Toxicol. 2019 May;42(3):243-251. doi: 10.1080/01480545.2017.1416625. Epub 2018 Jan 17.
The toxicity of aflatoxins results in cancer and liver disease. Several natural substances such as plants exhibited their ability to inhibit the initiation of aflatoxin carcinogenesis. The aim of this study was to evaluate the effect of Alchornea cordifolia on biomarkers in an aflatoxin B1 (AFB1) exposed rats. The contents of polyphenols, flavonoids and the antioxidant activity of A. cordifolia ethanolic leaf extract (EELac) were assessed. Groups of rats were treated orally with a daily dose of a mixture of AFB1 at a dose of 150 μg/kg body weight and EELac (50, 100 and 300 mg/kg body weight) for 21 days. Biomarkers of AFB1, such as the AFB1-lysine adduct and aflatoxin M1 were assayed in blood and urine, respectively, using an HPLC system with a fluorescence detector. The contents of polyphenols and flavonoids were 6783.23 ± 272.76 μg EAG/g and 10.54 ± 3.15% of dry matter, respectively. EELac showed a good antioxidant activity (IC50 = 12.65 ± 0.13 μg/mL). The administration of the mixture (AFB1 + EELac) at different doses significantly reduced the level of AFB1-lysine adduct from 14.04 ± 2.1 to 4.13 ± 0.9 ng/mg albumin and that of Aflatoxin M1 (AFM1) from 456 ± 16 to 220 ± 24 ng/mL (p <0.05). The rate of reduction was 70.58% for AFB1-lysine adduct and 51.75% for AFM1. A. cordifolia could be used in the prevention of toxicity induced by AFB1 on account of its high content in phenolic compounds.
黄曲霉毒素的毒性会导致癌症和肝脏疾病。一些天然物质如植物表现出抑制黄曲霉毒素致癌作用起始的能力。本研究的目的是评估心叶山麻杆对黄曲霉毒素B1（AFB1）暴露大鼠生物标志物的影响。评估了心叶山麻杆乙醇叶提取物（EELac）中多酚、黄酮类化合物的含量以及抗氧化活性。将大鼠分组，每天口服剂量为150μg/kg体重的AFB1与EELac（50、100和300mg/kg体重）的混合物，持续21天。分别使用带荧光检测器的高效液相色谱系统检测血液和尿液中AFB1的生物标志物，如AFB1-赖氨酸加合物和黄曲霉毒素M1。多酚和黄酮类化合物的含量分别为6783.23±272.76μg EAG/g和干物质的10.54±3.15%。EELac表现出良好的抗氧化活性（IC50 = 12.65±0.13μg/mL）。不同剂量的混合物（AFB1 + EELac）给药显著降低了AFB1-赖氨酸加合物水平，从14.04±2.1降至4.13±0.9ng/mg白蛋白，黄曲霉毒素M1（AFM1）水平从456±16降至220±24ng/mL（p <0.05）。AFB1-赖氨酸加合物的降低率为70.58%，AFM1为51.75%。由于心叶山麻杆中酚类化合物含量高，可用于预防AFB1诱导的毒性。
