Gashi Goneta, Mahovlić Vesna, Manxhuka-Kerliu Suzana, Podrimaj-Bytyqi Arjeta, Gashi Luljeta, Elezaj Isa R
a Department of Pathology, Faculty of Medicine , University of Prishtina , Prishtina , Kosovo.
b Department of Pathology and Cytology , Clinical Unit of Gynecological Cytology, University Hospital Center , Zagreb , Croatia.
Biomarkers. 2018 May-Jun;23(4):364-372. doi: 10.1080/1354750X.2018.1428828. Epub 2018 Jan 31.
The loss of genomic stability plays an important role in carcinogenesis. Therefore, it is imperative to use certain biomarkers of DNA damage due to genomic instability in order to predict cancer risk. The aim of this study was the evaluation of genomic instability in patients with cervical lesions.
We investigated the genetic damages in 80 subjects: 40 patients with high-grade squamous intraepithelial lesions (HSIL), 20 patients with invasive squamous cervical cancer (SCC) and 20 healthy women with a biomarker in two different tissues; the micronucleus (MN) test in peripheral blood lymphocytes (PBL), and in buccal exfoliated cells (BEC). This study also examined the frequency of other nuclear anomalies such as nucleoplasmic bridges (NPBs) and nuclear bunds (NBUDs) in PBL.
The frequency of MN in BEC, MN in PBL, NPB in PBL and NBUD in PBL were significantly higher (p < 0.001), in patients compared to controls. The DNA damages in BEC and PBL were correlated positively with histological grade of cervical lesions.
Although larger studies are needed, our data support the predictive value of MN, NPB and NBUD as biomarkers of genomic instability for evaluation of risk level of cancer diseases.
基因组稳定性的丧失在致癌过程中起着重要作用。因此，有必要使用某些因基因组不稳定导致的DNA损伤生物标志物来预测癌症风险。本研究的目的是评估宫颈病变患者的基因组不稳定性。
我们调查了80名受试者的基因损伤情况：40例高级别鳞状上皮内病变（HSIL）患者、20例浸润性宫颈鳞癌（SCC）患者和20名健康女性，在两种不同组织中检测生物标志物；对外周血淋巴细胞（PBL）和颊部脱落细胞（BEC）进行微核（MN）试验。本研究还检测了PBL中其他核异常的频率，如核质桥（NPB）和核芽（NBUD）。
与对照组相比，患者BEC中的MN频率、PBL中的MN频率、PBL中的NPB频率和PBL中的NBUD频率显著更高（p < 0.001）。BEC和PBL中的DNA损伤与宫颈病变的组织学分级呈正相关。
尽管需要更大规模的研究，但我们的数据支持MN、NPB和NBUD作为基因组不稳定生物标志物对评估癌症疾病风险水平的预测价值。
