Center for Addiction Research (CARe), Department of Psychiatry, Socialpsychiatry and Psychotherapy, Hannover Medical School, Hannover, Germany.
Department of Addictive Behavior and Addiction Medicine, Central Institute of Mental Health, Medical Faculty Mannheim/Heidelberg University, Mannheim, Germany.
Eur Addict Res. 2017;23(6):306-311. doi: 10.1159/000486279. Epub 2018 Jan 16.
Atrial natriuretic peptide (ANP) is well known in psychiatric disorders to modulate hypothalamic-pituitary-adrenal axis activity. Disturbances of ANP have been described in early abstinent alcohol-dependent patients. This is the first longitudinal investigation on cytosine-phosphatidyl-guanine (CpG)-island promoter methylation of the ANP gene in the blood of tobacco-dependent patients.
In a longitudinal approach, we investigated whether changes in ANP serum levels correlated to CpG methylation of the respective gene promoters on days 1, 7, and 14 of tobacco withdrawal.
Compared to non-smokers, promoter-related deoxyribonucleic acid methylation of the ANP promoter was significantly elevated on days 7 and 14 of withdrawal in tobacco-dependent patients. Baseline methylation status of the ANP promoter was not significantly different from controls, arguing for an impaired regulation during withdrawal.
心房利钠肽（ANP）在精神疾病中被广泛认为可以调节下丘脑-垂体-肾上腺轴的活动。在早期戒断的酒精依赖患者中，已经描述了 ANP 的紊乱。这是首次在烟草依赖患者的血液中对 ANP 基因的胞嘧啶-磷酸-鸟嘌呤（CpG）岛启动子甲基化进行的纵向研究。
采用纵向研究方法，我们研究了 ANP 血清水平的变化是否与烟草戒断第 1、7 和 14 天相应基因启动子的 CpG 甲基化相关。
与非吸烟者相比，在烟草依赖患者戒断的第 7 和第 14 天，ANP 启动子相关的脱氧核糖核酸甲基化明显升高。ANP 启动子的基线甲基化状态与对照组无显著差异，提示在戒断期间存在调节障碍。
