Department of Physical and Rehabilitation Medicine, Stem Cell and Regenerative Medicine Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
J Cell Physiol. 2018 Aug;233(8):6196-6206. doi: 10.1002/jcp.26475. Epub 2018 Mar 7.
Tendon derived stem cells (TDSCs) have been used as a therapeutic agent and as a healing marker. However, there has been no study about the characteristics of TDSCs extracted from tendinopathic tendon tissues. The aim of this study was to find the different characteristics of TDSCs according to the factors to induce the tendinopathy. Five- and fifteen-week old Sprague Dawley rats were used for this study and chemically-induced and injury-induced tendinopathy models were made depending on the age of the animal for different types of tendinopathy. TDSCs from chemically-induced tendinopathy showed markedly low proliferation compared to those from age-matched normal control and injury-induced tendinopathy. In addition, TDSCs from chemically-induced tendinopathy progressed to osteogenesis under an osteogenic differentiation environment more than those from other groups. In contrast, TDSCs from injury-induced tendinopathy showed markedly high proliferation and high expression of type III collagen and α-SMA compared to other groups. Adipogenic potentials in TDSCs from injury-induced tendinopathy were also higher. These different characteristics might be helpful in the development new therapeutic agents for tendon regeneration according to different factors to induce the tendinopathy.
肌腱衍生干细胞（TDSCs）已被用作治疗剂和愈合标记物。然而，目前还没有研究从腱病性肌腱组织中提取的 TDSCs 的特征。本研究旨在根据诱导腱病的因素，寻找 TDSCs 的不同特征。本研究使用 5 周和 15 周龄的 Sprague Dawley 大鼠，并根据动物的年龄制作了化学诱导和损伤诱导的腱病模型，以产生不同类型的腱病。与年龄匹配的正常对照组和损伤诱导的腱病相比，化学诱导的腱病中的 TDSCs 增殖明显较低。此外，在成骨分化环境下，来自化学诱导的腱病的 TDSCs 比其他组更容易向成骨分化。相比之下，来自损伤诱导的腱病的 TDSCs 的增殖明显较高，III 型胶原和α-SMA 的表达也较高。来自损伤诱导的腱病的 TDSCs 的成脂潜能也较高。这些不同的特征可能有助于根据诱导腱病的不同因素开发新的腱再生治疗剂。
