Kataoka T, Broek D, Wigler M
Cell. 1985 Dec;43(2 Pt 1):493-505. doi: 10.1016/0092-8674(85)90179-5.
We have cloned CYR1, the S. cerevisiae gene encoding adenylate cyclase. The DNA sequence of CYR1 can encode a protein of 2026 amino acids. This protein would contain a central region comprised of over twenty copies of a 23 amino acid repeating unit with remarkable homology to a 24 amino acid tandem repeating unit of a trace human serum glycoprotein. Gene disruption and biochemical experiments indicate that the catalytic domain of adenylate cyclase resides in the carboxyl terminal 400 amino acids. Elevated expression of adenylate cyclase suppresses the lethality that otherwise results from loss of RAS gene function in yeast. Yeast adenylate cyclase, made in E. coli, cannot be activated by added RAS protein.
我们已经克隆了CYR1，即酿酒酵母中编码腺苷酸环化酶的基因。CYR1的DNA序列可编码一个含有2026个氨基酸的蛋白质。该蛋白质将包含一个中央区域，该区域由23个氨基酸重复单元的二十多个拷贝组成，与一种微量人血清糖蛋白的24个氨基酸串联重复单元具有显著的同源性。基因破坏和生化实验表明，腺苷酸环化酶的催化结构域位于羧基末端的400个氨基酸中。腺苷酸环化酶的高表达抑制了酵母中RAS基因功能丧失否则会导致的致死性。在大肠杆菌中产生的酵母腺苷酸环化酶不能被添加的RAS蛋白激活。
