Chen Xiao, Zhang Hongling, Wan Yanjian, Chen Xi, Li Yuanyuan
School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
College of Health Science and Nursing, Wuhan Polytechnic University, Wuhan, China.
PLoS One. 2018 Jan 17;13(1):e0191149. doi: 10.1371/journal.pone.0191149. eCollection 2018.
2,4-Dichlorophenoxyacetic acid (2,4-D) is a chlorophenoxy herbicide used worldwide. We describe a high-performance liquid chromatography (HPLC) method with UV detection for the determination of 2,4-D in female and male rat serum. This allows to observe the change of serum 2,4-D concentration in rats with time and its pharmacokinetics characteristics with a simple, rapid, optimized and validated method. The serum samples are pretreated and introduced into the HPLC system. The analytes are separated in a XDB-C18 column with a mobile phase of acetonitrile (solvent A) and 0.02 M ammonium acetate (containing 0.1% formic acid) (solvent B) using a gradient elution at a flow rate of 1.0 mL/min. The wavelength for UV detection was set at 230 nm. Calibration curve for 2,4-D was constructed over a range of 0.1-400 mg/L. The method was successfully applied to study the pharmacokinetics of 2,4-D in rats in this study. After oral administration of 300 mg/kg and 60 mg/kg 2,4-D, the mean Cmax values were 601.9 and 218.4 mg/L, the AUC0→∞ values were 23,722 and 4,127 mg×h/L and the clearance (Cl) were 1.10 and 0.02 L/(h×kg), respectively. The developed method was found to be specific, precise, reproducible and rapid.
2,4-二氯苯氧乙酸（2,4-D）是一种在全球范围内使用的氯苯氧基除草剂。我们描述了一种采用紫外检测的高效液相色谱（HPLC）方法，用于测定雌性和雄性大鼠血清中的2,4-D。这使得我们能够通过一种简单、快速、优化且经过验证的方法观察大鼠血清中2,4-D浓度随时间的变化及其药代动力学特征。血清样品经过预处理后引入HPLC系统。分析物在XDB-C18柱中分离，流动相为乙腈（溶剂A）和0.02 M醋酸铵（含0.1%甲酸）（溶剂B），采用梯度洗脱，流速为1.0 mL/min。紫外检测波长设定为230 nm。2,4-D的校准曲线在0.1 - 400 mg/L范围内构建。该方法在本研究中成功应用于研究2,4-D在大鼠体内的药代动力学。口服给予300 mg/kg和60 mg/kg的2,4-D后，平均Cmax值分别为601.9和218.4 mg/L，AUC0→∞值分别为23,722和4,127 mg×h/L，清除率（Cl）分别为1.10和0.02 L/(h×kg)。所建立的方法具有特异性、精密度高、可重复性好且快速的特点。
