National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2018 Jan 14;19(1):243. doi: 10.3390/ijms19010243.
The tetraploid species is cultivated widely throughout the world with high yield and moderate fiber quality, but its genetic basis is narrow. A set of 107 introgression lines (ILs) was developed with an interspecific cross using . 4105 as the recurrent parent and as the donor parent. A specific locus amplified fragment sequencing (SLAF-seq) strategy was used to obtain high-throughput single nucleotide polymorphism (SNP) markers. In total, 3157 high-quality SNP markers were obtained and further used for identification of quantitative trait loci (QTLs) for fiber quality and yield traits evaluated in multiple environments. In total, 74 QTLs were detected that were associated with five fiber quality traits (30 QTLs) and eight yield traits (44 QTLs), with 2.02-30.15% of the phenotypic variance explained (PVE), and 69 markers were found to be associated with these thirteen traits. Eleven chromosomes in the A sub-genome (At) harbored 47 QTLs, and nine chromosomes in the D sub-genome (Dt) harbored 27 QTLs. More than half (44 QTLs = 59.45%) showed positive additive effects for fiber and yield traits. Five QTL clusters were identified, with three in the At, comprised of thirteen QTLs, and two in the Dt comprised of seven QTLs. The ILs developed in this study and the identified QTLs will facilitate further molecular breeding for improvement of Upland cotton in terms of higher yield with enhanced fiber quality.
四倍体物种在世界各地广泛种植，具有高产和中等纤维质量，但遗传基础狭窄。利用种间杂交，以 4105 为轮回亲本， 为供体亲本，开发了一套 107 条导入系（ILs）。采用特异扩增片段测序（SLAF-seq）策略获得高通量单核苷酸多态性（SNP）标记。共获得 3157 个高质量 SNP 标记，并进一步用于鉴定在多个环境中评估的纤维质量和产量性状的数量性状位点（QTLs）。共检测到与五个纤维质量性状（30 个 QTLs）和八个产量性状（44 个 QTLs）相关的 74 个 QTLs，解释了 2.02-30.15%的表型方差（PVE），并发现 69 个标记与这些 13 个性状相关。A 亚基因组（At）的 11 条染色体携带 47 个 QTL，D 亚基因组（Dt）的 9 条染色体携带 27 个 QTL。超过一半（44 个 QTLs=59.45%）对纤维和产量性状表现出正加性效应。鉴定到五个 QTL 簇，其中三个位于 At，包含 13 个 QTLs，两个位于 Dt，包含 7 个 QTLs。本研究中开发的 ILs 和鉴定的 QTLs 将有助于进一步进行分子育种，以提高陆地棉的产量和改善纤维质量。
