State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Senegalese River Valley Development Agency (SAED), Saint-Louis Bp74, Senegal.
Int J Mol Sci. 2018 Feb 1;19(2):441. doi: 10.3390/ijms19020441.
Fiber quality and yield improvement are crucial for cotton domestication and breeding. With the transformation in spinning techniques and multiplicity needs, the development of cotton fiber quality and yield is of great importance. A genetic map of 5178 Single Nucleotide Polymorphism (SNP) markers were generated using 277 F population, from an intra-specific cross between two upland cotton accessions, CCRI35 a high fiber quality as female and Nan Dan Ba Di Da Hua (NH), with good yield properties as male parent. The map spanned 4768.098 cM with an average distance of 0.92 cM. A total of 110 Quantitative Traits Loci (QTLs) were identified for 11 traits, but only 30 QTLs were consistent in at least two environments. The highest percentage of phenotypic variance explained by a single QTL was 15.45%. Two major cluster regions were found, cluster 1 (chromosome17-D03) and cluster 2 (chromosome26-D12). Five candidate genes were identified in the two QTL cluster regions. Based on GO functional annotation, all the genes were highly correlated with fiber development, with functions such as protein kinase and phosphorylation. The five genes were associated with various fiber traits as follows: linked to qFM-D03_cb, , , associated with qFS-D12_cb and linked to qFY-D12_cb. Further structural annotation and fine mapping is needed to determine the specific role played by the five identified genes in fiber quality and yield related pathway.
纤维品质和产量的提高对于棉花的驯化和培育至关重要。随着纺纱技术的转变和多样性需求的增加，棉花纤维品质和产量的发展非常重要。利用 277 个 F1 群体，通过两个陆地棉品种 CCRI35（高纤维品质，作为母本）和 Nan Dan Ba Di Da Hua（NH，产量性状好，作为父本）进行种内杂交，生成了 5178 个单核苷酸多态性（SNP）标记的遗传图谱。图谱覆盖了 4768.098cM，平均距离为 0.92cM。共鉴定到 11 个性状的 110 个数量性状位点（QTL），但只有 30 个 QTL 在至少两个环境中一致。单个 QTL 解释的表型方差最高百分比为 15.45%。发现了两个主要的聚类区域，簇 1（染色体 17-D03）和簇 2（染色体 26-D12）。在这两个 QTL 聚类区域中鉴定到 5 个候选基因。根据 GO 功能注释，所有基因都与纤维发育高度相关，具有蛋白激酶和磷酸化等功能。这 5 个基因与各种纤维性状有关：与 qFM-D03_cb 相关，与 qFS-D12_cb 相关，与 qFY-D12_cb 相关。需要进一步进行结构注释和精细定位，以确定这 5 个鉴定基因在纤维品质和产量相关途径中的具体作用。
