Cai Cheng-Fu, Liu Cun-Shan, Shang-Guan Han-Jing, Yang Cai-Hong, Luo Xian-Yang, Shen Dong-Yan, Yang Shu-Yu
Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.
Biobank, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.
Oncol Lett. 2017 Dec;14(6):7896-7902. doi: 10.3892/ol.2017.7194. Epub 2017 Oct 16.
The aberrant expression of retinoic acid receptor-α (RARα) has been reported in various types of cancer. However, its association with the prognosis and development of laryngeal squamous cell carcinoma (LSCC) has not yet been determined. Therefore, the present study aimed to examine the expression and function of RARα in patients with LSCC. The expression of RARα in LSCC tissues was investigated using immunostaining. An MTT assay and flow cytometry analysis were also performed to investigate the function of RARα in the proliferation and cell cycle of LSCC cells. The expression of RARα was significantly elevated in LSCC tissues compared with adjacent noncancerous tissues (78.1 vs. 6.3%, P<0.05). The overexpression of RARα was associated with poorly differentiated features of LSCC (P<0.05). Furthermore, the downregulation of RARα inhibited the proliferation of LSCC cells, and arrested the cell cycle at the G1 phase via upregulation of cyclin dependent kinase inhibitor 1A, which may be associated with inhibition of the protein kinase B signaling pathway. Therefore, the overexpression of RARα may contribute to the development of LSCC through the regulation of the cell cycle. The results of the present study provide evidence that RARα serves an important function in LSCC development and may be a potential therapeutic target or prognostic predictor for LSCC.
已有报道称维甲酸受体-α（RARα）在多种癌症中存在异常表达。然而，其与喉鳞状细胞癌（LSCC）的预后及发展的相关性尚未确定。因此，本研究旨在检测LSCC患者中RARα的表达及功能。采用免疫染色法研究LSCC组织中RARα的表达。还进行了MTT试验和流式细胞术分析，以研究RARα在LSCC细胞增殖和细胞周期中的功能。与相邻的非癌组织相比，LSCC组织中RARα的表达显著升高（78.1%对6.3%，P<0.05）。RARα的过表达与LSCC的低分化特征相关（P<0.05）。此外，RARα的下调抑制了LSCC细胞的增殖，并通过上调细胞周期蛋白依赖性激酶抑制剂1A使细胞周期停滞在G1期，这可能与蛋白激酶B信号通路的抑制有关。因此，RARα的过表达可能通过调节细胞周期促进LSCC的发展。本研究结果提供了证据，表明RARα在LSCC发展中起重要作用，可能是LSCC的潜在治疗靶点或预后预测指标。
