Golubeva Yelena G, Warner Andrew C
Cancer Genomic Research Laboratory (CGR), Division of Cancer Epidemiology and Genetics, NCI, FNLCR, Leidos Biomedical Research, Inc, Gaithersburg, MD, USA.
Pathology-Histotechnology Laboratory (PHL), Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Methods Mol Biol. 2018;1723:33-93. doi: 10.1007/978-1-4939-7558-7_3.
Laser Capture Microdissection has earned a permanent place among modern techniques connecting histology and molecular biology. Laser Capture Microdissection has become an invaluable tool in medical research as a means for collection of specific cell populations isolated from their environment. Such genomic sample enrichment dramatically increases the sensitivity and precision of downstream molecular assays used for biomarker discovery, monitoring disease onset and progression, and in the development of personalized medicine. The diversity of research targets (cancerous and precancerous lesions in clinical and animal research, cell pellets, rodent embryos, frozen tissues, archival repository slides, etc.) and scientific objectives present a challenge in establishing standard protocols for Laser Capture Microdissection. In the present chapter, we share our experiences in design and successful execution of numerous diverse microdissection projects, and provide considerations to be taken into account in planning a microdissection study. Our workflow and protocols are standardized for a wide range of animal and human tissues and adapted to downstream analysis platforms.
激光捕获显微切割技术在连接组织学与分子生物学的现代技术中占据了一席之地。作为从其所处环境中分离特定细胞群体的一种手段，激光捕获显微切割技术已成为医学研究中一项不可或缺的工具。这种基因组样本富集显著提高了用于生物标志物发现、监测疾病发生和进展以及个性化医疗开发的下游分子检测的灵敏度和精确度。研究目标的多样性（临床和动物研究中的癌性和癌前病变、细胞沉淀、啮齿动物胚胎、冷冻组织、存档库玻片等）以及科学目标给建立激光捕获显微切割的标准方案带来了挑战。在本章中，我们分享在设计和成功执行众多不同显微切割项目方面的经验，并提供在规划显微切割研究时需考虑的因素。我们的工作流程和方案针对广泛的动物和人类组织进行了标准化，并适用于下游分析平台。
