Remy Sophie, Fulcrand Hélène, Labarbe Benoît, Cheynier Véronique, Moutounet Michel
INRA-Institut des Produits de la Vigne, Unité de Recherches Biopolymères et Arômes, 2 Place Viala, F-34060 Montpellier Cedex 2, France.
J Sci Food Agric. 2000 May 1;80(6):745-751. doi: 10.1002/(SICI)1097-0010(20000501)80:6<745::AID-JSFA611>3.0.CO;2-4.
Analysis of wine fractions before and after thiolysis confirmed the occurrence in red wine of direct reactions between anthocyanins and tannins established earlier in model solutions. Results showed the presence of two types of structures differing in the linkage position of the anthocyanin moiety. On one hand, detection of malvidin-3-glucoside (Mv3g) among thiolysis products revealed the presence of anthocyanin-derived pigments in which Mv3g is linked by its C-6 or C-8 top. On the other hand, LC/MS analysis allowed the detection of two derivatives tentatively identified as flavenes or a bicyclic condensation products yielded by the reaction of a flavanol monomer (C-6 or C-8 top) with malvidin-3-glucoside (C-4 position). The presence of the corresponding benzylthioethers after thiolysis of the polymeric fractions confirmed that procyanidins are similarly involved in the latter reaction. Besides, MS also allowed the detection of new benzylthioethers of catechin derivatives released after thiolysis of the wine fractions, indicating that in addition to the postulated processes other types of reactions take place in wines. © 2000 Society of Chemical Industry.
硫解前后葡萄酒组分的分析证实了在红酒中花色苷与单宁之间直接反应的发生，这一反应在之前的模型溶液中就已得到证实。结果显示存在两种结构类型，其花色苷部分的连接位置不同。一方面，硫解产物中检测到的甲基花青素-3-葡萄糖苷（Mv3g）表明存在源自花色苷的色素，其中Mv3g通过其C-6或C-8位连接。另一方面，液相色谱/质谱分析能够检测到两种衍生物，初步鉴定为黄烷或由黄烷醇单体（C-6或C-8位）与甲基花青素-3-葡萄糖苷（C-4位）反应生成的双环缩合产物。聚合物组分硫解后相应苄硫醚的存在证实了原花青素同样参与了后一反应。此外，质谱还能检测到葡萄酒组分硫解后释放的儿茶素衍生物的新苄硫醚，这表明除了假定的过程外，葡萄酒中还发生其他类型的反应。© 2000化学工业协会。
