Morrison E Heather, Bremner H Allan, Purslow Peter P
Department of Dairy and Food Science, The Royal Danish Agricultural and Veterinary University, DK-1958 Frederiksberg, Denmark.
Danish Institute for Fisheries Research, Department of Seafood Research, Technical University, DK-2800 Lyngby, Denmark.
J Sci Food Agric. 2000 May 1;80(6):691-697. doi: 10.1002/(SICI)1097-0010(20000501)80:6<691::AID-JSFA590>3.0.CO;2-T.
The cytoskeletal proteins actin, nebulin, spectrin, desmin, vinculin and talin were labelled immunohistochemically in sections of muscle from commercially available pigs and cod (Gadus morhua) taken pre-rigor and from samples stored for several days. Actin, nebulin and spectrin gave similar labelling patterns in both pork and cod muscle which remained the same in stored samples. Desmin was intensely labelled at the cell boundaries and within the body of the cells in both pork and cod in the initial and the stored samples. Vinculin was readily labelled in pork muscle but showed only diffuse labelling in fish. Labelling for talin in pork muscle was intense at the sarcolemma but was not present in samples stored for 4 days. In contrast, the label for talin was concentrated at the myotendinous junction of the cod muscle throughout the storage period. These are the first reports of the detection and location of spectrin and vinculin in fish muscle and of the location of talin. The results are discussed in terms of muscle structure, function and post-mortem tenderisation. © 2000 Society of Chemical Industry.
