Dornfest B S, Naughton B A, Lapin D M, Gordon A S
J Surg Oncol. 1985 Jul;29(3):187-92. doi: 10.1002/jso.2930290312.
A compensated hemolytic state was induced in rats by injection of phenylhydrazine (PHZ) over a 6-week period. The liver and kidney were perfused to determine the levels and time of appearance of a hepatic erythropoietic factor (HEF) which induces the production of hepatic erythropoietin (Ep) and its antagonist, a renal inhibitory factor (RIF). Erythropoietin assays on the perfusates have been previously reported. The amount of HEF in perfusates recovered from the livers of the PHZ-treated rats was significantly higher during the 4th to 5th weeks of treatment, coinciding with the time of increased liver production of Ep. During the 6th week of PHZ treatment, the titer of RIF in perfusates recovered from the kidney was markedly increased and the HEF titer was decreased to near the control level, suggesting inhibitory action of RIF on HEF synthesis and/or effect. These findings indicate that an HEF/RIF control mechanism regulates hepatic Ep production in this compensated hemolytic condition.
在6周的时间里，通过给大鼠注射苯肼（PHZ）诱导其进入代偿性溶血状态。对肝脏和肾脏进行灌注，以确定诱导肝脏产生促红细胞生成素（Ep）的肝脏促红细胞生成因子（HEF）及其拮抗剂——肾脏抑制因子（RIF）的水平和出现时间。此前已有关于灌注液中促红细胞生成素测定的报道。在治疗的第4至5周，从接受PHZ治疗的大鼠肝脏中回收的灌注液中HEF的量显著增加，这与肝脏Ep产生增加的时间一致。在PHZ治疗的第6周，从肾脏回收的灌注液中RIF的滴度显著增加，而HEF滴度降至接近对照水平，提示RIF对HEF合成和/或作用具有抑制作用。这些发现表明，在这种代偿性溶血状态下，一种HEF/RIF控制机制调节肝脏Ep的产生。
