Laboratory of Soil Microbiology and Calorimetry, Corporación Colombiana de Investigación Agropecuaria - Corpoica, Centro de Investigación Tibaitatá - kilómetro 14 vía Mosquera-Bogotá, Cundinamarca, Colombia.
Laboratory of CIMA, University of Nariño, Pasto, Colombia.
J Appl Microbiol. 2018 May;124(5):1175-1194. doi: 10.1111/jam.13698. Epub 2018 Feb 26.
This research aims to assess total-cadmium soil content and microbiological aspects to understand the dynamics of culturable cadmium-tolerant bacteria (CdtB) in cacao soils from northeastern Colombia.
An integration of inverted dish plating, Cd determination and a microcalorimetry assay (IMC) was carried out. A farm in Boyacá showed the highest level of total soil Cd (3·74 mg kg ) followed by farms in Santander and Arauca (2·76 and 1·16 mg kg , respectively). Coefficient of determination between total soil Cd and CFU of CdtB was high (R = 0·83) for the farm in Boyacá. Moreover, a pool of 129 CdtB was isolated, and phylogeny of 21 CdtB was discussed. Among CdtB strains isolated, Enterobacter sp. CdDB41 showed major Cd immobilization capacity (Q of 2·21 and 2·32 J at 6 and 24 mg l of CdCl ), with an immobilization rate of 0·220 mg kg h .
Among CdtB strains isolated, Enterobacter sp. CdDB41 showed major Cd immobilization capacity (Q of 2·21 and 2·32 J at 6 and 24 mg l of CdCl ), with an immobilization rate of 0·220 mg kg h .
Nothing is known about soil CdtB in cacao. Our data showed that CdtB such as Enterobacter sp. has high immobilization capacity. Furthermore, the otavite found in situ might be mineralized due to the bacterial metabolic activity of CdtB.
本研究旨在评估土壤总镉含量和微生物学方面的情况，以了解哥伦比亚东北部可可土中可培养镉耐受细菌（CdtB）的动态变化。
采用倒扣平板法、镉测定和微量量热法（IMC）进行了综合研究。博亚卡的一个农场显示出最高的土壤总镉含量（3.74mgkg），紧随其后的是桑坦德和阿劳卡的农场（分别为 2.76 和 1.16mgkg）。博亚卡农场土壤总镉与 CdtB 的 CFU 之间的决定系数很高（R=0.83）。此外，还分离出了 129 株 CdtB，并对 21 株 CdtB 的系统发育进行了讨论。在所分离的 CdtB 菌株中，肠杆菌属 CdDB41 表现出较强的 Cd 固定能力（在 6 和 24mgL 的 CdCl下 Q 值分别为 2.21 和 2.32J），固定率为 0.220mgkg h。
在所分离的 CdtB 菌株中，肠杆菌属 CdDB41 表现出较强的 Cd 固定能力（在 6 和 24mgL 的 CdCl下 Q 值分别为 2.21 和 2.32J），固定率为 0.220mgkg h。
目前尚不清楚可可土中的土壤 CdtB 情况。我们的数据表明，像肠杆菌属这样的 CdtB 具有很强的固定能力。此外，由于 CdtB 的细菌代谢活性，原位发现的 Otavite 可能被矿化。
