Codorean E, Gabrielescu E
Morphol Embryol (Bucur). 1985 Oct-Dec;31(4):269-74.
Cathepsin B was cytochemically investigated in the cells of synovial membranes and in the cell pellet of synovial fluids obtained from 50 patients with rheumatoid arthritis and eight patients with various nonrheumatoid arthropathies. The activity of Cathepsin B was estimated by using the substrate N-alpha-benzoyl-DL-arginine-naphthylamide HCl and diazoic dye Fast Corinth V in phosphate buffer pH 6.0 in the presence of EDTA and cysteine. A significant activity of cathepsin B was shown in lining mesothelial cells, in macrophages of the submesothelial infiltrations, as well as in fibroblasts prominent in the deep areas of rheumatoid synovial membranes. In the cell pellets of synovial fluids the highest activity of cathepsin B was found in the macrophages and polymorphonuclear leukocytes, accompanied by a variable activity in lymphocytes. The considerable activity of cathepsin B, an enzyme with degradative action upon collagen and proteoglycans, in the main cellular populations of rheumatoid synovial membranes and fluids, suggests its involvement in the genesis and maintenance of rheumatoid lesions.
采用细胞化学方法对50例类风湿性关节炎患者和8例各种非类风湿性关节病患者的滑膜细胞及滑膜液细胞沉淀中的组织蛋白酶B进行了研究。在pH 6.0的磷酸盐缓冲液中，于存在乙二胺四乙酸（EDTA）和半胱氨酸的条件下，使用底物N-α-苯甲酰-DL-精氨酸萘酰胺盐酸盐和重氮染料固红紫来评估组织蛋白酶B的活性。结果显示，在衬里间皮细胞、间皮下浸润的巨噬细胞以及类风湿性滑膜深层区域突出的成纤维细胞中，组织蛋白酶B具有显著活性。在滑膜液的细胞沉淀中，巨噬细胞和多形核白细胞中的组织蛋白酶B活性最高，淋巴细胞中的活性则各不相同。组织蛋白酶B是一种对胶原蛋白和蛋白聚糖具有降解作用的酶，在类风湿性滑膜及其液体的主要细胞群体中具有相当高的活性，这表明它参与了类风湿性病变的发生和维持过程。
