Gerstoft J, Dickmeiss E, Mathiesen L
Scand J Immunol. 1985 Nov;22(5):463-70. doi: 10.1111/j.1365-3083.1985.tb01904.x.
Lymphocytes from 16 AIDS patients were tested in the cell-mediated lympholysis assay (CML). The ability to produce alloreactive cytotoxic lymphocytes in vitro was found to be substantially reduced when compared with concomitantly investigated normal controls. Addition of interleukin 2 (IL-2) to the inducer cultures increased the cytotoxic activity, but not to normal levels. The CML response did not correlate with the relative or absolute number of Leu 3+ cells or the proliferation in effector suspensions. The ability to produce cytotoxic cells in CML, and the degree of potentiation by IL-2, was positively correlated with the absolute number of Leu 2+ cells in peripheral blood of the patients, which was below normal in 56% of the patients. It is suggested that the low CML in AIDS patients is primarily caused by defective T-cell help. In addition patients with decreased absolute numbers of Leu 2+ cells may have a reduced number of CTL precursors. The natural killer (NK) activity of AIDS lymphocytes was reduced, but could be improved by incubation with IL-2 in vitro. The mononuclear cells from the patients showed a decreased ability to respond and to stimulate in the mixed lymphocyte culture. In one of the AIDS patients, the CML was found to induce autoreactivity in vitro.
对16例艾滋病患者的淋巴细胞进行了细胞介导的淋巴细胞溶解试验（CML）。与同时研究的正常对照相比，发现体外产生同种异体反应性细胞毒性淋巴细胞的能力显著降低。在诱导培养物中添加白细胞介素2（IL-2）可增加细胞毒性活性，但未达到正常水平。CML反应与Leu 3+细胞的相对或绝对数量或效应悬浮液中的增殖无关。CML中产生细胞毒性细胞的能力以及IL-2的增强程度与患者外周血中Leu 2+细胞的绝对数量呈正相关，56%的患者该数量低于正常水平。提示艾滋病患者CML降低主要是由于T细胞辅助功能缺陷所致。此外，Leu 2+细胞绝对数量减少的患者可能具有较少的细胞毒性T淋巴细胞（CTL）前体。艾滋病淋巴细胞的自然杀伤（NK）活性降低，但体外与IL-2孵育可使其改善。患者的单核细胞在混合淋巴细胞培养中的反应和刺激能力降低。在1例艾滋病患者中，发现CML在体外可诱导自身反应性。
