Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Korea.
Department of Physical Medicine & Rehabilitation, Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Korea.
Photochem Photobiol. 2018 May;94(3):583-588. doi: 10.1111/php.12887. Epub 2018 Mar 2.
Previous studies have reported that 450 nm blue light emitting diode (BLED) induces apoptosis through a mitochondria-mediated pathway in cancer cells and reduces the early stage tumor growth. This study was performed to determine the effects of BLED-irradiated cell metabolites on cell injury. Our results showed that conditioned medium (CM) from cells irradiated with low-dose BLED (LCM) inhibited apoptosis and increased cell survival. Cell protection-related proteins were identified in cell metabolites of CM and LCM using 2-DE and MALDI-TOF analysis. Treatment with LCM inhibited apoptotic cell death and increased the live cell population. The cellular protective effect of LCM was associated with keratin and collagen type VI secretion from cells after low dose of BLED irradiation. Interestingly, expression of endoplasmic reticulum stress proteins was dose dependently increased after 4 h BLED irradiation. Only levels of BiP, CHOP and ERO1-Lα were decreased significantly after 24 h incubation, indicating their anti-apoptotic property in these cells. These results indicated that cell metabolites stimulated by low-dose BLED irradiation have a cytoprotective effect on cell injury via increasing transient intracellular ER stress. Further studies remain to provide the molecular mechanisms of LCM for cytoprotective activity.
先前的研究报告指出，450nm 蓝光发光二极管（BLED）通过线粒体介导的途径在癌细胞中诱导细胞凋亡，并减少早期肿瘤生长。本研究旨在确定 BLED 照射细胞代谢物对细胞损伤的影响。我们的结果表明，低剂量 BLED（LCM）照射细胞的条件培养基（CM）抑制细胞凋亡并增加细胞存活率。使用 2-DE 和 MALDI-TOF 分析鉴定了 CM 和 LCM 中的细胞代谢物中的细胞保护相关蛋白。用 LCM 处理可抑制细胞凋亡性死亡并增加活细胞群体。LCM 的细胞保护作用与低剂量 BLED 照射后细胞角质蛋白和胶原 VI 型的分泌有关。有趣的是，内质网应激蛋白的表达在 4 小时 BLED 照射后呈剂量依赖性增加。只有 BiP、CHOP 和 ERO1-Lα 的水平在 24 小时孵育后显著降低，表明它们在这些细胞中有抗凋亡作用。这些结果表明，低剂量 BLED 照射刺激的细胞代谢物通过增加短暂的细胞内质网应激对细胞损伤具有细胞保护作用。进一步的研究仍然需要提供 LCM 对细胞保护活性的分子机制。
