Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Jeonbuk Regional Cyclotron Research Center & Biotracer Imaging Lab, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, 20, Geonji-ro, Duckjin-gu, Jeonju-si, Jeollabuk-do, 561-803, South Korea.
Lasers Med Sci. 2021 Feb;36(1):99-109. doi: 10.1007/s10103-020-03018-x. Epub 2020 May 4.
We have previously reported the protective effects of blue light-emitting diode (BLED)-stimulated cell metabolites on cell injury. To further examine the effect of conditioned media (CM) derived from BLED (5 J/cm)-exposed human normal fibroblasts (CMBL5) for clinical application, we have used the choline chloride and phenol red-free media and then concentrated CMBL5 using a centrifugal filter unit. The collected CMBL5-lower part (CMBL5-LO) has evaluated the inflammatory protein expression profile in LPS-stimulated RAW264.7 cells. Comprehensive metabolomic profiling of CMBL5-LO was carried out using hybrid tandem mass spectrometry. Treatment with CMBL5-LO showed the cytoprotective effect on apoptotic cell death, but rather increased apoptotic cells after treatment with CMBL5-upper part (CMBL5-UP). In addition, CMBL5-LO inhibited several chemo-attractants, including interleukin (IL)-6, macrophage inflammatory protein (MIP)-2, chemokine (C-C motif) ligand 5 (CCL5), granulocyte colony-stimulating factor (GCSF), and monocyte chemoattractant protein-1 (MCP-1) expression. Pro-inflammatory nitric oxide was decreased after CMBL5-LO treatment, but not by CMBL5-UP treatment. Interestingly, treatment with CMBL5-LO stimulated expression of heme oxygenase-1, indicating its anti-inflammatory property. Most endoplasmic reticulum (ER) stress proteins except for transcription factor C/EBP homologous protein (CHOP) were highly expressed after irradiation with BLED in cells. Further studies are needed to examine the precise mechanism by CMBL5-LO in cells.
我们之前报道过蓝光发光二极管（BLED）刺激的细胞代谢物对细胞损伤的保护作用。为了进一步研究源自 BLED（5 J/cm）暴露的人正常成纤维细胞的条件培养基（CMBL5）在临床应用中的效果，我们使用了无胆碱氯化物和酚红的培养基，然后使用离心过滤装置浓缩 CMBL5。收集的 CMBL5 下部（CMBL5-LO）评估了 LPS 刺激的 RAW264.7 细胞中炎症蛋白表达谱。使用混合串联质谱法对 CMBL5-LO 进行了全面的代谢组学分析。CMBL5-LO 处理表现出对细胞凋亡死亡的细胞保护作用，但在处理 CMBL5 上部（CMBL5-UP）后反而增加了凋亡细胞。此外，CMBL5-LO 抑制了几种趋化因子，包括白细胞介素（IL）-6、巨噬细胞炎症蛋白（MIP）-2、趋化因子（C-C 基序）配体 5（CCL5）、粒细胞集落刺激因子（GCSF）和单核细胞趋化蛋白-1（MCP-1）的表达。CMBL5-LO 处理后促炎的一氧化氮减少，但 CMBL5-UP 处理后没有减少。有趣的是，CMBL5-LO 处理刺激血红素加氧酶-1 的表达，表明其具有抗炎作用。在细胞中用 BLED 照射后，除转录因子 C/EBP 同源蛋白（CHOP）外，大多数内质网（ER）应激蛋白的表达都很高。需要进一步研究以检查 CMBL5-LO 在细胞中的精确作用机制。
