National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, 271018, China.
National Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, 271018, China.
BMC Plant Biol. 2018 Jan 20;18(1):18. doi: 10.1186/s12870-018-1232-6.
The roles in photosystem I (PSI) assembly of the nucleus-encoded thylakoid protein Y3IP1 who interacts with the plastid-encoded Ycf3 protein that has been well-characterized in plants. However, its function and potential mechanisms in other aspects remain poorly understood.
We identified the apple MdY3IP1 gene, which encodes a protein highly homologous to the Arabidopsis Y3IP1 (AtY3IP1). Ectopic expression of MdY3IP1 triggered early-flowering and enhanced salt tolerance in Arabidopsis plants. MdY3IP1 controlled floral transition by accelerating sugar metabolism process in plant cells, thereby influencing the expression of flowering-associated genes. The increase in salt stress tolerance in MdY3IP1-expressing plants correlated with reduced reactive oxygen species (ROS) accumulation, and an increase in lateral root development by regulating both auxin biosynthesis and transport, as followed by enhancement of salt tolerance in Arabidopsis. Overall, these findings provide new evidences for additional functions of Y3IP1-like proteins and their underlying mechanisms of which Y3IP1 confers early-flowering and salt tolerance phenotypes in plants.
These observations suggest that plant growth and stress resistance can be affected by the regulation of the MdY3IP1 gene. Further molecular and genetic approaches will accelerate our knowledge of MdY3IP1 functions in PSI complex formation and plants stress resistance, and inform strategies for creating transgenic crop varieties with early maturity and high-resistant to adverse environmental conditions.
在光系统 I（PSI）组装中，细胞核编码的类囊体蛋白 Y3IP1 与已在植物中得到充分研究的质体编码的 Ycf3 蛋白相互作用。然而，其在其他方面的功能和潜在机制仍知之甚少。
我们鉴定了苹果 MdY3IP1 基因，该基因编码的蛋白与拟南芥 Y3IP1（AtY3IP1）高度同源。MdY3IP1 的异位表达可引发拟南芥的早花和增强耐盐性。MdY3IP1 通过加速植物细胞中的糖代谢过程来控制花发育的转变，从而影响与开花相关的基因的表达。在表达 MdY3IP1 的植物中，耐盐性的提高与活性氧（ROS）积累的减少有关，通过调节生长素的生物合成和运输，促进侧根发育，从而增强了拟南芥的耐盐性。总的来说，这些发现为 Y3IP1 类蛋白的其他功能及其潜在机制提供了新的证据，Y3IP1 赋予了植物早花和耐盐表型。
这些观察结果表明，MdY3IP1 基因的调控可以影响植物的生长和抗逆性。进一步的分子和遗传方法将加速我们对 MdY3IP1 在 PSI 复合物形成和植物抗逆性中的功能的了解，并为创建具有早熟和高抗逆性的转基因作物品种提供信息。
