Institute of Polymers, Bulgarian Academy of Sciences, Akad. G. Bonchev Street 103A, 1113, Sofia, Bulgaria; Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Street, 1000, Sofia, Bulgaria.
Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Street, 1000, Sofia, Bulgaria.
Carbohydr Polym. 2018 Mar 1;183:165-172. doi: 10.1016/j.carbpol.2017.12.016. Epub 2017 Dec 11.
We report a novel approach for fabricating nanocomposite polysaccharide-based carriers for sustained delivery of poorly-water-soluble drugs by embedding stabilized core-shell micelles (SPM) possessing hydrophobic cores into super-macroporous hydroxypropyl cellulose (HPC) cryogels. Firstly, nano-sized SPM were synthesized by loading and photochemical crosslinking of pentaerythritoltetraacrylate (PETA) in poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) (PEOPPOPEO) core-shell micelles. Next, HPC cryogels containing different amount of SPM were fabricated by combination of cryogenic treatment and photo-crosslinking. A crosslinking agent, N,N'-methylenebisacrylamide, was used to enhance the density of polymer network. The effect of SPM concentration on gel fraction yield, swelling degree, cryogel morphology and mechanical properties were studied. Nanocomposite cryogels were loaded with curcumin and their encapsulation efficiency and drug release profile as a function of SPM content were investigated. The cytotoxic effect of blank and curcumin loaded nanocomposite cryogels was assessed as well.
我们报告了一种新的方法，通过将具有疏水性内核的稳定核壳型胶束（SPM）嵌入超微孔羟丙基纤维素（HPC）冷冻凝胶中，来制备用于持续递送疏水性差的药物的纳米复合多糖载体。首先，通过负载和光化学交联五丙烯酸季戊四醇酯（PETA）于聚（氧化乙烯）-b-聚（氧化丙烯）-b-聚（氧化乙烯）（PEOPPOPEO）核壳型胶束中来合成纳米级 SPM。接下来，通过冷冻处理和光交联结合制备了含有不同 SPM 含量的 HPC 冷冻凝胶。使用交联剂 N，N'-亚甲基双丙烯酰胺来增强聚合物网络的密度。研究了 SPM 浓度对凝胶分数产率、溶胀度、冷冻凝胶形态和机械性能的影响。纳米复合冷冻凝胶被负载姜黄素，并研究了 SPM 含量对其包封效率和药物释放曲线的影响。还评估了空白和负载姜黄素的纳米复合冷冻凝胶的细胞毒性。
