Programa de Pós-graduação em Bioquímica e Biologia Molecular, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil; Programa de Pós-graduação em Ciências da Saúde, Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Programa de Pós-graduação em Bioquímica e Biologia Molecular, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil; Faculdade de Ciências da Saúde do Trairi, Universidade Federal do Rio Grande do Norte, Santa Cruz, RN, Brazil.
Carbohydr Polym. 2018 Mar 1;183:192-200. doi: 10.1016/j.carbpol.2017.12.018. Epub 2017 Dec 13.
The occurrence of a natural and unmodified highly sulfated chondroitin sulfate from Litopenaeus vannamei heads (sCS) is herein reported. Its partial digestion by Chondroitinases AC and ABC together with its electrophoretic migration profile revealed it as a highly sulfated chondroitin sulfate despite its average molecular weight being similar to CSA. Using orthogonal 1D/2D NMR experiments, the anomeric signals (δ 4.62/106.0) corresponding to unusual 2,3-di-O-Sulfo-GlcA (∼36%), U3 (δ 4.42/84.1, ∼63%) and U2 (4.12/80.1, ∼50%) substitutions were confirmed. In addition, non-sulfated GlcA (δ 4.5/106.3) linked to 4-O- (A1, 36%) or 6-O-Sulfo (A1, 28%) GalNAc (δ 4.64/103.5) was observed. Although the biological role of sCS in shrimp is unknown, its influence on hemostasis was also demonstrated. The sCS identification brings to light new questions about the hierarchical model of GAGs biosynthesis and contributes to the better understanding of the subtle relationship between GAGs structure and function.
本文报道了一种天然未修饰的、富含磺酸基的凡纳滨对虾头软骨素硫酸盐（sCS）的存在。其经软骨素酶 AC 和 ABC 的部分消化及其电泳迁移谱表明，尽管其平均分子量与 CSA 相似，但它是一种高度硫酸化的软骨素硫酸盐。通过正交 1D/2D NMR 实验，确定了具有异常 2,3-二-O-磺酸基-GlcA（约 36%）、U3（δ 4.42/84.1，约 63%）和 U2（4.12/80.1，约 50%）取代的端基信号（δ 4.62/106.0）。此外，观察到非磺酸化的 GlcA（δ 4.5/106.3）与 4-O-（A1，36%）或 6-O-磺酸基（A1，28%）GalNAc（δ 4.64/103.5）相连。尽管 sCS 在虾中的生物学作用尚不清楚，但它对止血的影响也得到了证实。sCS 的鉴定揭示了 GAG 生物合成层次模型的新问题，并有助于更好地理解 GAG 结构与功能之间的微妙关系。
