Biothecnology and Genetic Center, State University of Santa Cruz, Ilhéus, BA, Brazil.
Federal University of Viçosa, Viçosa, MG, Brazil.
Appl Microbiol Biotechnol. 2018 Mar;102(5):2235-2249. doi: 10.1007/s00253-018-8759-1. Epub 2018 Jan 20.
The cell surface of Toxoplasma gondii is covered by antigens (SAGs) from the SRS family anchored by glycosylphosphatidylinositol (GPI) and includes antigens from the SAG2 family. Among these, the SAG2A surface antigen shows great potential in activating humoral responses and has been used in characterizing the acute phase of infection and in the serological diagnosis of toxoplasmosis. In this study, we aimed to evaluate rSAG2A-induced proteins in BALB/c and C57BL/c mice macrophages and to evaluate the phenotypic polarization induced in the process. We treated the peritoneal macrophages from mouse strains that were resistant or susceptible to T. gondii with rSAG2A to analyze their proteomic profile by mass spectrometry and systems biology. We also examined the gene expression of these cells by RT-qPCR using the phenotypic markers of M1 and M2 macrophages. Differences were observed in the expression of proteins involved in the inflammatory process in both resistant and susceptible cells, and macrophages were preferentially induced to obtain a pro-inflammatory immune response (M1) via the overexpression of IL-1β in mice susceptible to this parasite. These data suggest that the SAG2A antigen induces phenotypic and classical activation of macrophages in both resistant and susceptible strains of mice during the acute phase of the disease.
刚地弓形虫的细胞表面被糖基磷脂酰肌醇（GPI）锚定的 SRS 家族抗原（SAGs）覆盖，其中包括 SAG2 家族抗原。在这些抗原中，SAG2A 表面抗原在激活体液免疫反应方面具有巨大潜力，已被用于描述感染的急性期和弓形虫病的血清学诊断。在这项研究中，我们旨在评估 rSAG2A 在 BALB/c 和 C57BL/c 小鼠巨噬细胞中诱导的蛋白，并评估在此过程中诱导的表型极化。我们用 rSAG2A 处理对弓形虫有抵抗力或易感的小鼠品系的腹腔巨噬细胞，通过质谱和系统生物学分析其蛋白质组图谱。我们还使用 M1 和 M2 巨噬细胞的表型标志物通过 RT-qPCR 检查这些细胞的基因表达。在有抵抗力和易感的细胞中，参与炎症过程的蛋白表达存在差异，并且通过过度表达易感于这种寄生虫的小鼠中的 IL-1β，诱导巨噬细胞优先获得促炎免疫反应（M1）。这些数据表明，在疾病的急性期，SAG2A 抗原诱导了两种品系小鼠巨噬细胞的表型和经典激活。
