Nosratabadi M, Kordbacheh P, Kachuei R, Safara M, Rezaie S, Afshari Ma
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Curr Med Mycol. 2017 Jun;3(2):13-19. doi: 10.18869/acadpub.cmm.3.2.13.
Keratinophilic fungi are among the important groups of fungi living in the soil. This study aimed to isolate and identify keratinophilic fungi from the soil of three Iranian islands, namely Greater Tunb, Abu Musa, and Sirri, located in the Persian Gulf using morphological and molecular (polymerase chain reaction) methods.
In this study, a total of 60 soil samples were collected from the three islands of Greater Tunb, Abu Musa, and Sirri. The samples were analyzed for the presence of the keratinophilic fungi using a hair baiting technique. Furthermore, the identification of keratinophilic fungi was accomplished through the employment of molecular and sequencing techniques.
A total of 130 fungal isolates, including 11 genera with 24 species, were collected. Accordingly, (24;18.5%), (17; 13.1%), species (15; 11.5%), species ( 8;6.1%), (8; 6.1%), species (8;6.1%), spp ( 6; 4.6%), species (5; 3.8%), (4;3.1%), (4; 3.1%), (4;3.1%), species (4; 3.1%), and other fungi( 23; 17.8 %) isolates were identified . All isolates of keratinophilic fungi were isolated from the soils with the pH range of 7-9.
The results of this study contributed towards a better conceptualization of the incidence pattern of keratinophilic fungi in the regions of Iran. Given that no study has investigated this issue, the findings of the present study can be beneficial for the management of public health surveillance, physicians, and epidemiologists.
嗜角质真菌是生活在土壤中的重要真菌类群之一。本研究旨在利用形态学和分子生物学（聚合酶链反应）方法，从位于波斯湾的伊朗三大岛屿——大通布岛、阿布穆萨岛和锡里岛的土壤中分离和鉴定嗜角质真菌。
在本研究中，共从大通布岛、阿布穆萨岛和锡里岛这三个岛屿采集了60份土壤样本。采用毛发诱饵技术分析样本中嗜角质真菌的存在情况。此外，通过分子和测序技术对嗜角质真菌进行鉴定。
共收集到130株真菌分离株，包括11个属的24个种。相应地，鉴定出了[具体属名1]（24株；18.5%）、[具体属名2]（17株；13.1%）、[具体种名3]（15株；11.5%）、[具体种名4]（8株；6.1%）、[具体属名5]（8株；6.1%）、[具体种名6]（8株；6.1%）、[具体属名7]（6株；4.6%）、[具体种名8]（5株；3.8%）、[具体属名9]（4株；3.1%）、[具体属名10]（4株；3.1%）、[具体属名11]（4株；3.1%）、[具体种名12]（4株；3.1%）以及其他真菌（23株；17.8%）分离株。所有嗜角质真菌分离株均从pH值为7 - 9的土壤中分离得到。
本研究结果有助于更好地了解伊朗地区嗜角质真菌的发生模式。鉴于此前尚无研究调查过此问题，本研究结果对公共卫生监测管理、医生和流行病学家可能会有所帮助。
