Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt.
Department of Clinical Pathology, Faculty of Medicine, Cairo University, Giza 12613, Egypt.
Molecules. 2022 Apr 14;27(8):2541. doi: 10.3390/molecules27082541.
Fungal-bacterial co-culturing is a potential technique for the production of secondary metabolites with antibacterial activity. Twenty-nine fungal species were screened in a co-culture with carbapenem-resistant at different temperatures. A temperature of 37 ° showed inhibition of bacterial growth. Antimicrobial susceptibility testing for was conducted to compare antibiotic resistance patterns before and after the co-culture. Genotypic comparison of the was performed using next generation sequencing (NGS). It was shown that two out of five , with sequence type ST 101 isolates, lost , , , and genes after the co-culture with fungus. The other three isolates (ST 383 and 147) were inhibited in the co-culture but did not show any changes in resistance. The total ethyl acetate extract of the fungal-bacterial co-culture was tested against using a disc diffusion method. The concentration of the crude extract was 0.97 mg/µL which resulted in total inhibition of the bacteria. Using chromatographic techniques, the purified compounds were identified as 11-octadecenoic acid, 2,4-Di-tert-butylphenol, 2,3-Butanediol and 9-octadecenamide. These were tested against using the well diffusion method at a concentration of 85 µg/µL which resulted in total inhibition of bacteria. The co-culture results indicated that bacteria under chemical stress showed variable responses and induced fungal secondary metabolites with antibacterial activities.
真菌-细菌共培养是一种生产具有抗菌活性的次生代谢产物的潜在技术。在与不同温度下的耐碳青霉烯的 共培养中筛选了 29 种真菌。在 37°C 的温度下显示出抑制细菌生长的作用。对 进行了抗生素敏感性测试，以比较共培养前后的抗生素耐药模式。使用下一代测序 (NGS) 对 进行了基因型比较。结果表明，在与 真菌共培养后，五个 中的两个，具有序列类型 ST101 的分离株，失去了 、 、 、 和 基因。另外三个分离株（ST383 和 147）在共培养中被抑制，但没有显示任何耐药性变化。用圆盘扩散法对真菌-细菌共培养的总乙酸乙酯提取物进行了 测试。粗提物的浓度为 0.97mg/µL，导致细菌完全抑制。使用色谱技术，鉴定出纯化合物为 11-十八烯酸、2,4-二叔丁基苯酚、2,3-丁二醇和 9-十八烯酰胺。在 85µg/µL 的浓度下，用孔扩散法对其进行了测试，结果导致细菌完全抑制。共培养结果表明，处于化学胁迫下的细菌表现出不同的反应，并诱导具有抗菌活性的真菌次生代谢产物。
