Kamari A, Sepahvand A, Mohammadi R
Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Curr Med Mycol. 2017 Jun;3(2):20-25. doi: 10.29252/cmm.3.2.20.
species are pathogenic and non-pathogenic basidiomycete yeasts that are found widely in the environment. Based on phenotypic methods, this genus has many species; however, its taxonomy is presently being re-evaluated by modern techniques. The species complex includes two sibling taxa of and . We aimed to investigate the possible distribution of species in pigeon nests and trees in Ilam, Iran, using molecular techniques.
Two hundred and seventy-four specimens were collected from pigeon nests and trees during 2016-2017. All the specimens were sub-cultured on Sabouraud Glucose Agar with chloramphenicol and bird seed agar. For molecular identification, the ITS15.8SITS2 rDNA region was amplified using the first and fourth internal transcribed spacer (ITS1 and ITS4, respectively) primers. The purified products were applied for cycle sequencing reactions in forward direction with ITS1 primer. The obtained results were analyzed with Chromas 2.3.
Thirty-three out of 186 cultures (17.7%) and 11 out of 88 cultures (12.5%) were positive among pigeon nest and tree specimens, respectively. (17.2%), (3.4%), (3.4%), (3.4%), and (3.4%) were isolated from pigeon nests, and (25%) was the only species isolated from trees.
The presence of pigeons and trees in the vicinity of some particular places such as rest homes and hospitals should be considered as a risk factor for the immunocompromised population.
某些担子菌酵母物种具有致病性，而另一些则无致病性，它们在环境中广泛存在。基于表型方法，该属有许多物种；然而，其分类目前正通过现代技术重新评估。某物种复合体包括两个姊妹分类群。我们旨在利用分子技术调查伊朗伊拉姆地区鸽巢和某树中该物种的可能分布情况。
2016 - 2017年期间从鸽巢和某树收集了274个标本。所有标本在含氯霉素的沙氏葡萄糖琼脂和鸟籽琼脂上进行传代培养。为进行分子鉴定，使用第一和第四内部转录间隔区引物（分别为ITS1和ITS4）扩增ITS1 - 5.8S - ITS2 rDNA区域。纯化产物用ITS1引物进行正向循环测序反应。所得结果用Chromas 2.3进行分析。
在鸽巢标本中，186个培养物中有33个（17.7%）呈阳性，在某树标本中，88个培养物中有11个（12.5%）呈阳性。从鸽巢中分离出某菌（17.2%）、另一菌（3.4%）、又一菌（3.4%）、再一菌（3.4%）和还有一菌（3.4%），从某树中分离出的唯一某物种是另一菌（25%）。
在疗养院和医院等一些特定场所附近存在鸽子和某树应被视为免疫功能低下人群的一个危险因素。
