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影响关键干细胞因子表达的翻译调控因子的蛋白质相互作用图谱

Protein Interaction Mapping of Translational Regulators Affecting Expression of the Critical Stem Cell Factor Nos.

作者信息

Malik Sumira, Jang Wijeong, Kim Changsoo

机构信息

Hormone Research Center, School of Biological Sciences and Technology, College of Natural Sciences,Chonnam National University, Gwangju 61186, Republic of Korea.

Dept. of Agriculture and Forestry, Tula's Institute Dhoolkot, Dehradun-248011, India.

出版信息

Dev Reprod. 2017 Dec;21(4):449-456. doi: 10.12717/DR.2017.21.4.449. Epub 2017 Dec 31.

DOI:10.12717/DR.2017.21.4.449
PMID:29354790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5769139/
Abstract

The germline stem cells of the ovary continuously produce eggs throughout the life- span. Intricate regulation of stemness and differentiation is critical to this continuous production. The translational regulator Nos is an intrinsic factor that is required for maintenance of stemness in germline stem cells. Nos expression is reduced in differentiating cells at the post-transcriptional level by diverse translational regulators. However, molecular mechanisms underlying Nos repression are not completely understood. Through three distinct protein-protein interaction experiments, we identified specific molecular interactions between translational regulators involved in Nos repression. Our findings suggest a model in which protein complexes assemble on the 3' untranslated region of Nos mRNA in order to regulate Nos expression at the post-transcriptional level.

摘要

卵巢的生殖系干细胞在整个生命周期中持续产生卵子。对干性和分化的精细调控对于这种持续产生至关重要。翻译调节因子Nos是维持生殖系干细胞干性所必需的内在因子。在转录后水平，多种翻译调节因子会使Nos在分化细胞中的表达降低。然而，Nos抑制的分子机制尚未完全明确。通过三项不同的蛋白质-蛋白质相互作用实验，我们确定了参与Nos抑制的翻译调节因子之间的特定分子相互作用。我们的研究结果提出了一个模型，即蛋白质复合物在Nos mRNA的3'非翻译区组装，以便在转录后水平调节Nos的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/50a6961e6248/dr-21-4-449-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/df70bc15a0ca/dr-21-4-449-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/3e08ba0cf3be/dr-21-4-449-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/50a6961e6248/dr-21-4-449-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/df70bc15a0ca/dr-21-4-449-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/3e08ba0cf3be/dr-21-4-449-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6940/5769139/50a6961e6248/dr-21-4-449-g3.jpg

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Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.
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Biosci Rep. 2019 Jun 4;39(6). doi: 10.1042/BSR20190099. Print 2019 Jun 28.
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Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9465-70. doi: 10.1073/pnas.1120473109. Epub 2012 May 29.
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