基于温度梯度的重组大肠杆菌生产丙酮酸氧化酶的高细胞密度补料分批发酵
Temperature gradient-based high-cell density fed-batch fermentation for the production of pyruvate oxidase by recombinant E. coli.
作者信息
Liang Jianguang, Zhao Jie, Wang Zejian, Wang Yonghong
机构信息
a School of Biology and Food Engineering , Changshu Institute of Technology , Changshu , PR China.
b State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , PR China.
出版信息
Prep Biochem Biotechnol. 2018 Feb 7;48(2):188-193. doi: 10.1080/10826068.2018.1425709. Epub 2018 Feb 16.
Pyruvate oxidase (PyOD) is a very powerful enzyme for clinical diagnostic applications and environmental monitoring. Influences of temperature on cell growth, plasmid stability, and PyOD expression during the PyOD fermentation process by recombinant Escherichia coli were investigated. Based on the influences of temperature on the physiological metabolism, a novel high-cell density fed-batch cultivation with gradient temperature decrease strategy for effective PyOD production was achieved, under which the biomass (OD) of recombinant E. coli could reach to 71 and the highest PyOD activity in broth could reach to 3,307 U/L in 26 hr fermentation.
丙酮酸氧化酶（PyOD）是一种在临床诊断应用和环境监测中非常强大的酶。研究了温度对重组大肠杆菌发酵生产PyOD过程中细胞生长、质粒稳定性和PyOD表达的影响。基于温度对生理代谢的影响，实现了一种新颖的采用梯度降温策略的高效PyOD生产的高细胞密度补料分批培养方法，在此方法下，重组大肠杆菌的生物量（OD）在26小时发酵过程中可达到71，发酵液中PyOD的最高活性可达3307 U/L。
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