评估土壤中酯酶活性的高通量方法。
High-throughput method for the evaluation of esterase activity in soils.
作者信息
Tsuboi Shun, Tanaka Takumi, Yamamoto-Tamura Kimiko, Kitamoto Hiroko
机构信息
Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), 305-8604 Tsukuba, Japan.
Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), 305-8604 Tsukuba, Japan.
出版信息
J Microbiol Methods. 2018 Mar;146:22-24. doi: 10.1016/j.mimet.2018.01.009. Epub 2018 Jan 31.
We describe a method to quickly evaluate soil esterase activity using p‑nitrophenyl valerate as the substrate. Unwanted coloration of the control samples was suppressed by cooling. Esterase activity can be evaluated using arbitrary amounts of soil. Sample dispensation was simplified and the number of examinations per soil sample reduced.
我们描述了一种使用戊酸对硝基苯酯作为底物快速评估土壤酯酶活性的方法。通过冷却抑制了对照样品不必要的显色。可以使用任意量的土壤评估酯酶活性。简化了样品分配，减少了每个土壤样品的检测次数。
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