使用单克隆抗体和金标抗体在组织切片中对细胞表面标志物进行电子显微镜定位。
Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies.
作者信息
Rooney N, Day C, Gray T, Underwood J C
出版信息
J Pathol. 1986 Jan;148(1):29-34. doi: 10.1002/path.1711480106.
PMID:2935607
Abstract
We describe a method for the ultrastructural localization of surface markers on cells in intact tissue sections. Using a primary monoclonal antibody to a surface marker followed by a second stage gold-labelled antibody, surface antigens can be specifically localized. This enables cellular interactions to be studied in solid tissues.
摘要
我们描述了一种在完整组织切片中对细胞表面标志物进行超微结构定位的方法。使用针对表面标志物的一抗，随后使用二抗金标抗体，可特异性定位表面抗原。这使得能够在实体组织中研究细胞间相互作用。
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