Van Thi Thu Hao, Anwar Arif, Scott Peter C, Moore Robert J
1 School of Science, RMIT University , Bundoora, Victoria, Australia .
2 Scolexia Pty Ltd. , Moonee Ponds, Victoria, Australia .
Foodborne Pathog Dis. 2018 Aug;15(8):526-530. doi: 10.1089/fpd.2017.2367. Epub 2018 Jan 22.
Campylobacter jejuni and Campylobacter coli play a major role in bacteria-related foodborne illness in humans. Recently, a newly identified species, Campylobacter hepaticus, was shown to be the causative agent of spotty liver disease in chickens. The pathogenic potential of C. hepaticus in humans is unknown. This new species contains genes usually used to detect C. jejuni and C. coli in DNA-based detection methods, such as the hippuricase (hipO) gene and the glyA (serine hydroxymethyltransferase) gene, with a high degree of similarity. Therefore, polymerase chain reaction (PCR) primers used to detect these species need to be evaluated carefully to prevent misidentification of these important Campylobacter species. A multiplex PCR was developed and optimized to simultaneously and specifically identify the presence of C. jejuni, C. coli, and C. hepaticus in chicken samples containing high-complexity microbiota. The assay represents a new diagnostic tool for investigating the epidemiology of Campylobacter colonization in poultry and environmental samples. It may also be applicable to the investigation of Campylobacter contamination in food and in outbreaks of campylobacteriosis.
空肠弯曲菌和结肠弯曲菌在人类细菌性食源性疾病中起主要作用。最近，一种新发现的菌种——肝弯曲菌，被证明是鸡斑点肝病的病原体。肝弯曲菌对人类的致病潜力尚不清楚。这种新菌种含有通常用于基于DNA的检测方法中检测空肠弯曲菌和结肠弯曲菌的基因，如马尿酸酶（hipO）基因和甘氨酸A（丝氨酸羟甲基转移酶）基因，相似度很高。因此，用于检测这些菌种的聚合酶链反应（PCR）引物需要仔细评估，以防止误认这些重要的弯曲菌菌种。开发并优化了一种多重PCR方法，以同时特异性地鉴定含有高度复杂微生物群的鸡肉样品中空肠弯曲菌、结肠弯曲菌和肝弯曲菌的存在。该检测方法是一种用于调查家禽和环境样品中弯曲菌定植流行病学的新诊断工具。它也可能适用于食品中弯曲菌污染以及弯曲菌病暴发的调查。
