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神经转录本的杂交组织化学

Hybridization Histochemistry of Neural Transcripts.

作者信息

Young W Scott, Song June, Mezey Éva

机构信息

Section on Neural Gene Expression, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland.

Adult Stem Cell Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.

出版信息

Curr Protoc Neurosci. 2018 Jan 22;82:1.3.1-1.3.27. doi: 10.1002/cpns.39.

DOI:10.1002/cpns.39
PMID:29357110
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6217960/
Abstract

This unit presents protocols to locate RNA transcripts in tissues. Numerous approaches are detailed, including those that use radiolabeled or colorimetric probes. Also, the probes may be modified oligodeoxynucleotides, singly or in pairs, as well as ribonucleic acids. High sensitivity and specificity are obtained, especially with sets of oligodeoxynucleotide pairs. © 2018 by John Wiley & Sons, Inc.

摘要

本单元介绍了在组织中定位RNA转录本的实验方案。详细阐述了多种方法，包括使用放射性标记或比色探针的方法。此外，探针可以是修饰的寡脱氧核苷酸，单链或双链均可，也可以是核糖核酸。特别是使用寡脱氧核苷酸对时，可获得高灵敏度和特异性。© 2018约翰威立国际出版公司。

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Hybridization Histochemistry of Neural Transcripts.神经转录本的杂交组织化学
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Hybridization Histochemistry of Neural Transcripts.神经转录本的杂交组织化学
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Detection and Quantification of Multiple RNA Sequences Using Emerging Ultrasensitive Fluorescent In Situ Hybridization Techniques.使用新兴的超灵敏荧光原位杂交技术检测和定量多种RNA序列
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Raphe serotonin neuron-specific oxytocin receptor knockout reduces aggression without affecting anxiety-like behavior in male mice only.中缝5-羟色胺神经元特异性催产素受体敲除仅在雄性小鼠中减少攻击行为而不影响焦虑样行为。
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