神经转录本的杂交组织化学
Hybridization Histochemistry of Neural Transcripts.
作者信息
Young W Scott, Song June, Mezey Éva
机构信息
Section on Neural Gene Expression, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland.
Adult Stem Cell Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.
出版信息
Curr Protoc Neurosci. 2018 Jan 22;82:1.3.1-1.3.27. doi: 10.1002/cpns.39.
Abstract
This unit presents protocols to locate RNA transcripts in tissues. Numerous approaches are detailed, including those that use radiolabeled or colorimetric probes. Also, the probes may be modified oligodeoxynucleotides, singly or in pairs, as well as ribonucleic acids. High sensitivity and specificity are obtained, especially with sets of oligodeoxynucleotide pairs. © 2018 by John Wiley & Sons, Inc.
摘要
本单元介绍了在组织中定位RNA转录本的实验方案。详细阐述了多种方法，包括使用放射性标记或比色探针的方法。此外，探针可以是修饰的寡脱氧核苷酸，单链或双链均可，也可以是核糖核酸。特别是使用寡脱氧核苷酸对时，可获得高灵敏度和特异性。© 2018约翰威立国际出版公司。
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