一种利用拉曼光谱法鉴别生乳中噬菌体和抗生素污染物的简单快速方法。
A simple and fast method for discrimination of phage and antibiotic contaminants in raw milk by using Raman spectroscopy.
作者信息
Acar-Soykut Esra, Tayyarcan Emine Kubra, Boyaci Ismail Hakki
机构信息
1Food Research Center, Hacettepe University, Beytepe, 06800 Ankara, Turkey.
2Department of Food Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey.
出版信息
J Food Sci Technol. 2018 Jan;55(1):82-89. doi: 10.1007/s13197-017-2798-y. Epub 2017 Nov 9.
Phage and antibiotic in raw milk poses significant risks for starter culture activity in fermented products. Therefore, rapid detection of phage and antibiotic contaminations in raw milk is a crucial process in dairy science. For this purpose, a preliminary novel method for detection of phage and antibiotic was developed by using Raman spectroscopy. phages and ampicillin which are quite important elements in dairy industry were used as model. The phage and antibiotic samples were added to raw milk separately, and Raman measurements were carried out. The obtained spectra were processed with a chemometric method. In this study, it has been demonstrated that the presence of phage has a titer sufficient to stop the fermentation (10 pfu/ml), and antibiotic in a concentration which inhibits the growth of starter cultures (0.5 µg/ml) in raw milk could be discriminated through Raman spectroscopy with a short analysis time (30 min).
生乳中的噬菌体和抗生素对发酵产品中发酵剂的活性构成重大风险。因此，快速检测生乳中的噬菌体和抗生素污染是乳品科学中的一个关键过程。为此，利用拉曼光谱开发了一种初步的噬菌体和抗生素检测新方法。以乳品工业中非常重要的噬菌体和氨苄青霉素为模型。将噬菌体和抗生素样品分别添加到生乳中，并进行拉曼测量。所得光谱采用化学计量学方法进行处理。在本研究中，已证明噬菌体的存在具有足以阻止发酵的效价（10 pfu/ml），并且通过拉曼光谱在短分析时间（30分钟）内可以区分生乳中抑制发酵剂生长的浓度（0.5μg/ml）的抗生素。
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