Serrano Luis A, Yang Ye, Salvati Elisa, Stellacci Francesco, Krol Silke, Guldin Stefan
Department of Chemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Chem Commun (Camb). 2018 Feb 6;54(12):1485-1488. doi: 10.1039/c7cc07668a.
At present, drug dosage is based on standardised approaches that disregard pharmakokinetic differences between patients and lead to non-optimal efficacy and unnecessary side effects. In this work, we demonstrate the potential of pH-mediated fluorescence spectroscopy for therapeutic drug monitoring in complex media. We apply this principle to the simultaneous quantification of the chemotherapeutic prodrug Irinotecan and its active metabolite SN-38 from human plasma across the clinically relevant concentration range, i.e. from micromolar to nanomolar at molar ratios of up to 30 : 1.
目前，药物剂量是基于标准化方法确定的，这些方法忽略了患者之间的药代动力学差异，导致疗效不理想和出现不必要的副作用。在这项工作中，我们展示了pH介导的荧光光谱法在复杂介质中进行治疗药物监测的潜力。我们将这一原理应用于同时定量测定人血浆中化疗前药伊立替康及其活性代谢物SN-38，其浓度范围涵盖临床相关浓度，即从微摩尔到纳摩尔，摩尔比高达30∶1。
