Voĭtsitskiĭ V M, Dyl' K A
Ukr Biokhim Zh (1978). 1986 Jan-Feb;58(1):79-82.
The method of dynamic capacity in the model organic phase-water system was used to investigate a possibility of studying the electrical function of Ca2+,Mg2+-ATPase from sarcoplasmic reticulum of the rabbit hind limb skeletal muscles. Decane and decane solution of azolectin were used as an organic phase. It is stated that in the model systems the sarcoplasmic reticulum Ca2+,Mg2+-ATPase did not cause ATP-dependent changes in the boundary Volta potential (delta phi) irrespective of the presence of polyvalent cation chelates in the organic phase. The fragmented sarcoplasmic reticulum is able of realizing Mg-ATP, Ca2+-dependent generation of delta phi only with phospholipids present in the organic phase. It is supposed that generation of delta phi of the fragmented sarcoplasmic reticulum is due to the active transport of calcium ions by the reticulum Ca2+,Mg2+-ATPase.
采用模型有机相 - 水体系中的动态容量法，研究从兔后肢骨骼肌肌浆网中提取的Ca2 +，Mg2 + - ATP酶电功能的可能性。癸烷和卵磷脂的癸烷溶液用作有机相。结果表明，在模型系统中，无论有机相中是否存在多价阳离子螯合物，肌浆网Ca2 +，Mg2 + - ATP酶均不会引起边界伏打电位（δφ）的ATP依赖性变化。只有当有机相中存在磷脂时，破碎的肌浆网才能实现Mg - ATP、Ca2 +依赖性的δφ产生。据推测，破碎肌浆网的δφ产生是由于肌浆网Ca2 +，Mg2 + - ATP酶对钙离子的主动转运所致。
