Kurskii M D, Fedorov A N, Piskarev V B, Chanturiya A N
Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR, Kiev.
Biomed Sci. 1991;2(4):374-8.
Ca2+ ATPase was isolated from rabbit skeletal muscle sarcoplasmic reticulum and used to form structures resembling potential-dependent calcium channels within the membrane lipid bilayer of liposomes. The orientation of these structures in the bilayer was dependent on the conditions used for enzyme incorporation. The results obtained indicate that Ca2+ ATPase may be involved in the passive transport of calcium ions from the sarcoplasmic reticulum which may be regulated by the membrane potential. The membrane potential within the reticulum is probably positive at the moment of calcium ion release.
从兔骨骼肌肌浆网中分离出钙ATP酶，并用于在脂质体的膜脂双层内形成类似于电位依赖性钙通道的结构。这些结构在双层中的取向取决于用于酶掺入的条件。所得结果表明，钙ATP酶可能参与了肌浆网中钙离子的被动转运，而这可能受膜电位调节。在钙离子释放时，肌浆网内的膜电位可能为正。
