DExSI：一种用于快速定量分析 GC-MS 检测到的 13C 标记代谢物的新工具。
DExSI: a new tool for the rapid quantitation of 13C-labelled metabolites detected by GC-MS.
机构信息
Department of Biochemistry and Molecular Biology, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, Parkville, VIC 3010, Australia.
出版信息
Bioinformatics. 2018 Jun 1;34(11):1957-1958. doi: 10.1093/bioinformatics/bty025.
Abstract
SUMMARY
Stable isotope directed metabolomics is increasingly being used to measure metabolic fluxes in microbial, plant and animal cells. Incorporation of 13C/15N isotopes into a wide range of metabolites is typically determined using gas chromatography-mass spectrometry (GC/MS) or other hyphenated mass spectrometry approaches. The DExSI (Data Extraction for Stable Isotope-labelled metabolites) pipeline is an interactive graphical software package which can be used to rapidly quantitate isotopologues for a wide variety of metabolites detected by GC/MS. DExSI performs automated metabolite annotation, mass and positional isotopomer abundance determination and natural isotope abundance correction. It provides a range of output options and is suitable for high throughput analyses.
AVAILABILITY AND IMPLEMENTATION
DExSI is available for non-commercial use from: https://github.com/DExSI/DExSI/. For Microsoft Windows 7 or higher (64-bit).
CONTACT
malcolmm@unimelb.edu.au or michael.dagley@unimelb.edu.au.
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
摘要
摘要
稳定同位素示踪代谢组学正越来越多地被用于测量微生物、植物和动物细胞中的代谢通量。通过气相色谱-质谱联用仪 (GC/MS) 或其他联用质谱方法，通常可以确定各种代谢物中 13C/15N 同位素的掺入情况。DExSI（稳定同位素标记代谢物的数据提取）是一个交互式图形软件包，可用于快速定量 GC/MS 检测到的多种代谢物的同位素标记物。DExSI 可实现代谢物自动注释、质量和位置同位素丰度的测定以及自然同位素丰度的校正。它提供了多种输出选项，适用于高通量分析。
可用性和实现
DExSI 可从以下网址免费获取：https://github.com/DExSI/DExSI/。适用于 Microsoft Windows 7 或更高版本（64 位）。
联系人
malcolmm@unimelb.edu.au 或 michael.dagley@unimelb.edu.au。
补充信息
补充数据可在 Bioinformatics 在线获取。
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