西印度群岛特立尼达岛肉鸡中艾美耳球虫种类的分子鉴定
Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies.
作者信息
Brown Jordan Arianne, Blake Damer, Beard Jamila, Beharry Asha, Serrette Louanne, Soleyn Atlyn, Sookhoo Jamie, Blake Lemar, Brown Gabriel, Oura Christopher
机构信息
Department of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and Tobago.
Department of Pathobiology and Population Sciences, The Royal Veterinary College, Hertfordshire AL9 7TA, UK.
出版信息
Vet Sci. 2018 Jan 22;5(1):12. doi: 10.3390/vetsci5010012.
Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include , and . In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2-5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of (, and ) but not or . Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic species ( and ) was detected in feces taken from clinically sick birds sampled from the two pluck shops.
球虫病是一种对全球肉鸡产业具有重大经济影响的鸡肠道疾病。在鸡体内发现的球虫种类包括 、 和 。近年来，聚合酶链反应（PCR）已被开发用于准确快速地鉴定鸡的七种已知 种类。本研究的目的是使用种特异性实时PCR（qPCR）来鉴定特立尼达家禽中存在哪七种 种类。从特立尼达的6个肉鸡养殖场（每个养殖场2 - 5个鸡舍）收集了17份混合粪便样本。还从两个活禽市场（拔毛店）中表现出球虫病临床症状的鸟类中收集了粪便。qPCR检测到存在五种 （ 、 和 ），但未检测到 或 。在所有肉鸡养殖场都检测到了混合感染，并且在从两个拔毛店采集的临床患病鸟类粪便中检测到了两种高致病性 种类（ 和 ）的DNA。
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