Dewi Dias Aprita, Nugraheni Yudhi Ratna, Awaludin Aan, Ninditya Vika Ichsania, Priyowidodo Dwi, Nurcahyo Raden Wisnu, Ekawasti Fitrine, Prastowo Joko
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Departmenf of Animal Science, Politeknik Pembangunan Pertanian Yogyakarta Magelang, Magelang, Indonesia.
Open Vet J. 2025 Jan;15(1):139-150. doi: 10.5455/OVJ.2025.v15.i1.13. Epub 2025 Jan 31.
Coccidiosis caused by species (spp.) is a significant global health concern in goats leading to gastrointestinal illness. This condition causes clinical manifestations, including weight loss and diarrhea, resulting in worldwide economic losses. Subclinical symptoms can manifest during infection. Neglecting this disease can lead to severe morbidity and mortality. Therefore, addressing caprine coccidiosis is imperative.
This study aimed to determine the prevalence and molecular identification related to the natural infection of spp. in domestic goats originating from Java Island, Indonesia.
In total, fecal samples from 289 domestic goats were obtained across five provinces on Java Island, Indonesia: East Java, Central Java, D. I. Yogyakarta, West Java, and Banten. Morphological examinations were performed using the modified Whitlock method and saturated sugar flotation. Molecular assays targeting the 18S ribosomal ribonucleic acid have been employed for spp.-specific confirmation. Statistical analysis was performed using the Wilson binomial proportion and chi-square methods implemented in the online software.
A total of 92.7% (268/289) of fecal samples tested positive for spp. Phylogenetic tree analysis demonstrated that and closely resembled the reference sequences from China, Australia, and other countries.
This study identified and as the goat-infecting spp. of present on Java Island. The specific and accurate molecular identification conducted in this study will contribute to improved coccidiosis control and the development of effectiveness.
由球虫种类引起的球虫病是全球山羊健康的一个重大问题，会导致胃肠道疾病。这种疾病会引发包括体重减轻和腹泻在内的临床表现，造成全球经济损失。亚临床症状可在感染期间出现。忽视这种疾病会导致严重的发病率和死亡率。因此，应对山羊球虫病势在必行。
本研究旨在确定印度尼西亚爪哇岛家养山羊中与球虫种类自然感染相关的患病率及分子鉴定。
总共从印度尼西亚爪哇岛的五个省份收集了289份家养山羊的粪便样本：东爪哇、中爪哇、日惹特区、西爪哇和万丹。采用改良的惠特洛克方法和饱和蔗糖漂浮法进行形态学检查。针对18S核糖体核糖核酸的分子检测已用于球虫种类的特异性确认。使用在线软件中实施的威尔逊二项式比例法和卡方方法进行统计分析。
总共92.7%（268/289）的粪便样本检测出球虫种类呈阳性。系统发育树分析表明，[具体球虫种类]与来自中国、澳大利亚和其他国家的参考序列非常相似。
本研究确定[具体球虫种类]为爪哇岛存在的感染山羊的球虫种类。本研究进行的特异性和准确的分子鉴定将有助于改善球虫病控制及有效性的发展。
