Laboratory of Functional Plant Biology, Department of Biology, Faculty of Sciences, Ghent University, KL Ledeganckstraat 35, B-9000 Ghent, Belgium.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China; Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium.
Plant Sci. 2018 Mar;268:54-63. doi: 10.1016/j.plantsci.2017.12.005. Epub 2017 Dec 20.
Ultraviolet B light (UV-B, 280-315 nm) is the shortest wavelength of the solar spectrum reaching the surface of the Earth. It has profound effects on plants, ranging from growth regulation to severe metabolic changes. Low level UV-B mainly causes photomorphogenic effects while higher levels can induce stress, yet these effects tend to overlap. Here we identified a condition that allows growth reduction without obvious detrimental stress in wild type Arabidopsis rosette plants. This condition was used to study the effects of a daily UV-B dose on plant characteristics of UV-B adapted plants in detail. Exploration of the transcriptome of developing leaves indicated downregulation of genes involved in stomata formation by UV-B, while at the same time genes involved in photoprotective pigment biosynthesis were upregulated. These findings correspond with a decreased stomatal density and increased UV-B absorbing pigments. Gene ontology analysis revealed upregulation of defense related genes and meta-analysis showed substantial overlap of the UV-B regulated transcriptome with transcriptomes of salicylate and jasmonate treated as well as herbivore exposed plants. Feeding experiments showed that caterpillars of Spodoptera littoralis are directly affected by UV-B, while performance of the aphid Myzus persicae is diminished by a plant mediated process.
紫外线 B 光（UV-B，280-315nm）是到达地球表面的太阳光谱中最短的波长。它对植物有深远的影响，从生长调节到严重的代谢变化。低水平的 UV-B 主要引起光形态发生效应，而更高水平的 UV-B 可以诱导胁迫，但这些效应往往会重叠。在这里，我们确定了一种在野生型拟南芥莲座叶植物中不引起明显有害胁迫而减少生长的条件。利用这种条件，我们详细研究了每日 UV-B 剂量对 UV-B 适应植物的植物特性的影响。对发育中叶的转录组进行探索表明，UV-B 下调了与气孔形成有关的基因，而同时上调了与光保护色素生物合成有关的基因。这些发现与气孔密度降低和增加的 UV-B 吸收色素相对应。基因本体论分析显示防御相关基因的上调，荟萃分析显示 UV-B 调控的转录组与水杨酸和茉莉酸处理以及食草动物暴露植物的转录组有很大的重叠。饲养实验表明，海滨褐夜蛾的幼虫直接受到 UV-B 的影响，而烟粉虱的性能则受到植物介导的过程的削弱。
