Heare J Emerson, White Samuel J, Vadopalas Brent, Roberts Steven B
School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, United States of America.
PeerJ. 2018 Jan 15;6:e4261. doi: 10.7717/peerj.4261. eCollection 2018.
Olympia oysters are the only oyster native to the west coast of North America. The population within Puget Sound, WA has been decreasing significantly since the early 1900's. Current restoration efforts are focused on supplementing local populations with hatchery bred oysters. A recent study by Heare et al. (2017) has shown differences in stress response in oysters from different locations in Puget Sound however, nothing is known about the underlying mechanisms associated with these observed differences. In this study, expression of genes associated with growth, immune function, and gene regulatory activity in oysters from Oyster Bay, Dabob Bay, and Fidalgo Bay were characterized following temperature and mechanical stress. We found that heat stress and mechanical stress significantly changed expression in molecular regulatory activity and immune response, respectively. We also found that oysters from Oyster Bay had the most dramatic response to stress at the gene expression level. These data provide important baseline information on the physiological response of a to stress and provide clues to underlying performance differences in the three populations examined.
奥林匹亚牡蛎是北美洲西海岸唯一的本土牡蛎品种。自20世纪初以来,华盛顿州普吉特海湾内的该品种数量一直在显著减少。目前的恢复工作重点是用孵化场培育的牡蛎补充当地种群数量。Heare等人(2017年)最近的一项研究表明,普吉特海湾不同地点的牡蛎在应激反应方面存在差异,然而,对于这些观察到的差异背后的潜在机制却一无所知。在本研究中,对来自牡蛎湾、达博湾和菲达尔戈湾的牡蛎在温度和机械应激后与生长、免疫功能及基因调控活性相关的基因表达进行了表征。我们发现,热应激和机械应激分别显著改变了分子调控活性和免疫反应中的表达。我们还发现,来自牡蛎湾的牡蛎在基因表达水平上对应激的反应最为显著。这些数据提供了关于a对应激的生理反应的重要基线信息,并为所研究的三个种群之间潜在的性能差异提供了线索。