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重构的钙-ATP酶囊泡中双层膜去稳定化与二酰基甘油增强活性之间的相关性。

Correlation between bilayer destabilization and activity enhancement by diacylglycerols in reconstituted Ca-ATPase vesicles.

作者信息

Cheng K H, Hui S W

出版信息

Arch Biochem Biophys. 1986 Jan;244(1):382-6. doi: 10.1016/0003-9861(86)90127-x.

DOI:10.1016/0003-9861(86)90127-x
PMID:2936304
Abstract

Using the reconstituted Ca-ATPase vesicles as a model system, we demonstrated that the presence of 1,2-dioleoyl-sn-glycerol (diolein) in the membrane introduces a pronounced enhancement in the Ca-transport function of Ca-ATPase, while the 1,2-dipalmitoyl-sn-glycerol (dipalmitin) does not. We also found by both 31P NMR and freeze-fraction electron microscopy that diolein destabilized lipid bilayers to a greater extent than did dipalmitin. We conclude that the tendency of diacylglycerols to destabilize the phospholipid bilayer is related to their capacity to enhance the activity of the membrane calcium pump.

摘要

以重组的钙 - ATP酶囊泡作为模型系统，我们证明了膜中1,2 - 二油酰 - sn - 甘油（二油精）的存在显著增强了钙 - ATP酶的钙转运功能，而1,2 - 二棕榈酰 - sn - 甘油（二棕榈精）则没有。我们还通过31P核磁共振和冷冻断裂电子显微镜发现，二油精比二棕榈精更能使脂质双层不稳定。我们得出结论，二酰基甘油使磷脂双层不稳定的倾向与其增强膜钙泵活性的能力有关。

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