Ramautar Rawi
Division of Systems Biomedicine and Pharmacology, Leiden Academic Center for Drug Research, Leiden University, Leiden, The Netherlands.
Methods Mol Biol. 2018;1730:295-304. doi: 10.1007/978-1-4939-7592-1_22.
In the field of metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) can be considered a very useful analytical tool for the profiling of polar and charged metabolites. However, variability of migration time is an important issue in CE. An elegant way to minimize this problem is the use of non-covalently coated capillaries that is dynamic coating of the bare fused-silica capillary with solutions of charged polymers. In this protocol, an improved strategy for the profiling of cationic metabolites in urine by CE-MS using multilayered non-covalent capillary coatings is presented. Capillaries are coated with a bilayer of polybrene (PB) and poly(vinyl sulfonate) (PVS) or with a triple layer of PB, dextran sulfate (DS), and PB. The bilayer- and triple-layer-coated capillaries have a negative and positive outside layer, respectively. It is shown that the use of such capillaries provides very repeatable migration times.
在代谢组学领域，毛细管电泳-质谱联用（CE-MS）可被视为一种用于分析极性和带电代谢物的非常有用的分析工具。然而，迁移时间的变异性是毛细管电泳中的一个重要问题。一种巧妙的方法可以最小化这个问题，即使用非共价涂层毛细管，也就是用带电聚合物溶液对裸露的熔融石英毛细管进行动态涂层。在本方案中，提出了一种使用多层非共价毛细管涂层通过CE-MS分析尿液中阳离子代谢物的改进策略。毛细管先用聚凝胺（PB）和聚（乙烯磺酸盐）（PVS）双层涂层，或用PB、硫酸葡聚糖（DS）和PB三层涂层。双层和三层涂层毛细管的外层分别带负电和正电。结果表明，使用这种毛细管可提供非常可重复的迁移时间。
